MABS499

Anti-Phosphothreonine Antibody, clone RM102

clone RM102, from rabbit, purified by affinity chromatography

• Synonym(s): p-Thr, pthr, phospho-threonine
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: RM102, monoclonal
• Species reactivity: human
• Application: ICC, IHC, IP, WB
• Citations: -

Properties

• biological source: rabbit
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: RM102, monoclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): all
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
• isotype: IgG
• shipped in: wet ice
• target post-translational modification: phosphorylation (pThr)

Description

General description

Protein phosphorylation and dephosphorylation are basic mechanisms for the modification of protein function in eukaryotic cells. Phosphorylation is a rare post-translational event in normal tissue, however, the abundance of phosphorylated cellular proteins increases several fold following various activation processes which are mediated through phosphotyrosine, phosphoserine or phosphothreonine (p-tyr/p-ser/p-thr). Many signal transduction pathways, such as the EGF, PDGF and insulin receptor systems, contain tyr/ser/thr kinase which phosphorylate specific tyr/ser/thr residues upon binding of ligands to their receptors. T cell antigen receptor complex or the receptors for some hemopoietic growth factors may stimulate these phosphorylation associated kinases, and cells transformed by viral oncogenes contain elevated levels of phosphorylated tyr/ser/thr.

N/A kDa observed

Immunogen

Mix of recombinant protein and peptide corresponding to All Phosphothreonine.

Application

Immunocytochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on Calyculin A/Okadaic Acid treated A431 cells
Immunohistochemistry Analysis: A representative lot of this antibody at 1:500 dilution detected phosphothreonine modification on human colon cancer tissue
Immunoprecipitation Analysis: A representative lot of this antibody at 1:500 dilution immunoprecipitated phosphothreonine modified proteins from Calyculin A/Okadaic Acid treated A431 cell lysate

Anti-Phosphothreonine, clone RM102 is an antibody against Phosphothreonine for use in WB, ICC, IH, IP.

Analysis Note

Evaluated by Western Blotting on Calyculin A/Okadaic Acid treated HeLa cell lysate

Western Blotting Analysis: A 1:2000 dilution of this antibody detected phosphothreonine modification on Calyculin A/Okadaic Acid treated HeLa cell lysate

Other Notes

Concentration: Please refer to lot specific datasheet.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2