1.05438

Sabouraud Dextrose Agar

GranuCult^® prime, Dextrose 4 %, EP 5.6, USP 29, JP 4.05, granular, for yeasts, for molds, pack of 500 g and 5 kg

• Synonym(s): Fungus culture medium acc. to SABOURAUD, SABOURAUD 4% dextrose agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• Agency: EP 5.6; JP 4.05; USP 29
• Quality Level: 200
• form: granular
• composition: Dextrose, 4%
• manufacturer/tradename: GranuCult^® prime
• packaging: pack of 500 g and 5 kg
• technique(s): microbiological culture: suitable
• pH: 5.6 (25 °C, 65 g/L in H₂O, after autoclaving)
• solubility: 65 g/L
• bulk density: 680 kg/m³
• application(s): bioburden testing; industrial qc; microbiology; pharmaceutical
• storage temp.: 15-25°C
• suitability: molds; yeasts

Description

General description

Sabouraud 4% Dextrose Agar is a complex, granulated, dehydrated culture medium for cultivation and isolation of yeasts and molds. It also serves as the absence test for Candida albicans. The high concentration of dextrose and the low pH promotes the growth, the formation of spores (Conidia and Sporangia), and the formation of pigments of yeasts and molds. Additionally, it inhibits the growth of bacteria.

Application

Sabouraud 4% Dextrose Agar is designed for the determination of the total count of yeasts and molds in non-sterile products.

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 5.4 - 5.8
Typical composition (g/litre): Peptone from casein5.0; Peptone from meat 5.0; D(+)Glucose 40.0; Agar-agar 15.0.
Growth (Trichophyton mentagrophytes ATCC 18748): fair to very good
Growth (Trichophyton rubrum ATCC 28188): fair to good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Growth (Microsporum gallinae ATCC 12108): fair to very good
Growth (Microsporum canis ATCC 36299): good to very good
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Incubation: 7 days; 28°C
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Incubation: C. albicans and A. brasiliensis up to 5 daysat 20-25°C.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable