07-049

Anti-Nedd4

Upstate^®, from rabbit

• Synonym(s): E3 ubiquitin-protein ligase NEDD4, EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase NEDD4, Neural precursor cell expressed developmentally down-regulated protein 4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: IHC, WB
• Citations: 39

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: rat
• species reactivity (predicted by homology): human (based on 100% sequence homology)
• manufacturer/tradename: Upstate^®
• technique(s): immunohistochemistry: suitable (paraffin), western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_006145.2
• UniProt accession no.: P46934
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NDFIP1(80762)

Description

General description

E3 ubiquitin-protein ligase NEDD4 (UniProt: P46934; also known as EC: 2.3.2.26, Cell proliferation-inducing gene 53 protein, HECT-type E3 ubiquitin transferase Nedd4, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4) is encoded by the Nedd4 (also known as KIAA0093, Nedd4-1) gene (Gene ID: 4734) in human. NEDD4 is a peripheral membrane protein that is recruited to the plasma membrane by GRB10. Once complexed with GRB10 and IGF1R, it follows IGF1R internalization and remains associated with early endosomes. Nedd4 can accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. It is shown to specifically ubiquitinate Lysine 63 in target proteins. It also monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes. NEDD4 is reported to polyubiquitinate the epithelial sodium channel and any mutations that disrupt association between Nedd4 and sodium channel can lead to Liddle s syndrome, a hyperaldosteronism-like hypertension disease. NEDD4 can interact with viral proteins that contain a late-budding motif P-P-P-Y and this interaction is believed to be essential for viral particle budding of many retroviruses, like HTLV-1 Gag, and MLV Gag. It contains four WW domains (WW 1-4; aa 610-643; 767-800; 840-873; and 892-925) and one HECT domain (aa 984-1318). The WW domains mediate its interaction with PPxY motif-containing proteins.

~110 kDa observed; 102.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

GST/His-tagged recombinant fragment corresponding to 68 amino acids from the N-terminal half of human Nedd4.

Application

Anti-Nedd4 Antibody, Cat. No. 07-049, is a rabbit polyclonal antibody that detects Nedd4 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative detected NEDD44 in rat cerebral cortex, rat cerebellum, and human colon cancer tissue sections.

Biochem/physiol Actions

This rabbit polyclonal antibody specifically detects E3 ubiquitin-protein ligase Nedd4.

Physical form

Format: Purified

Purified rabbit polyclonal antibody in buffer containing 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 0.05% sodium azide.

Analysis Note

Control
Kidney cytoplasmic extracts.

Evaluated by Western Blotting in rat kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Nedd4 in rat kidney tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1