05-644

Anti-CAR Antibody, clone RmcB

clone RmcB, Upstate^®, from mouse

• Synonym(s): 46 kD coxsackievirus and adenovirus receptor (CAR) protein, CVB3 binding protein, CVB3-binding protein, Coxsackievirus B-adenovirus receptor, coxsackie virus B receptor, coxsackie virus and adenovirus receptor
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: RmcB, monoclonal
• species reactivity: human, rat, mouse
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001338
• UniProt accession no.: P78310
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CXADR(1525)

Description

General description

The coxsackievirus and adenovirus receptor (CAR) mediates cell attachment and infection by coxsackie B viruses and by a number of adenoviruses. CAR also mediates homotypic intercellular interactions. In polarized epithelial cells, CAR is closely associated with the tight junction, where it contributes to the barrier to paracellular flow of solutes and macromolecules. CAR′s biological roles are not well defined, but emerging evidence suggests that it may function during embryonic development and in regulating cell proliferation.

Specificity

Recognizes CAR at MW ~46 kDa.

Immunogen

Native human coxsackie virus-adenovirus receptor protein.

Application

Anti-CAR Antibody, clone RmcB detects level of CAR & has been published & validated for use in IC & WB.

Immunocytochemistry:
1-2 μg/mL of a previous lot showed positive immunostaining for CAR in HeLa cells, hCAR transfected CHO cells, but not in untransfected CHO cells fixed with 4% paraformaldehyde and permeabilized with 0.2% Triton-X.

Quality

Evaluated by Western Blot on mouse small intestine lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected Car on 10 µg of mouse small intestine lysates.

Target description

~46 kDa

Physical form

Format: Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl and 0.05% Sodium Azide, pH 7.4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1