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Supelco

Dextran

analytical standard, for GPC, 5,000

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About This Item

Linear Formula:
[C6H10O5]n
CAS Number:
9004-54-0
EC Number:
232-677-5
MDL number:
MFCD00130935
UNSPSC Code:
12352201
NACRES:
NA.24
Pricing and availability is not currently available.

grade

analytical standard
for GPC

Quality Level

100

form

powder or crystals

mol wt

Mn ~3,260
Mp ~4,440
Mw ~5,220

analyte chemical class(es)

oligosaccharides

technique(s)

gel permeation chromatography (GPC): suitable

Mw/Mn

~1.60

application(s)

food and beverages

SMILES string

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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General description

Dextran is a polysaccharide. Dextran fluids behave as colloids; and also are used as plasma substitution.[1] It may have siliconizing effect helping in coating raw serosal surfaces.[2]

Application


  • Wound healing applications: Dextran is used in collagen-polyurethane-dextran hydrogels, which enhance wound healing by inhibiting inflammation and promoting collagen fibrillogenesis, showcasing its importance in medical biomaterials (Aguayo-Morales et al., 2024).

  • Pharmaceutical research: Dextran is a key component in the formulation of pharmaceuticals, aiding in the stability and delivery of active pharmaceutical ingredients, which is crucial for the development of more effective therapeutic agents (Kohashi et al., 2024).

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL3769
BCCM4247
BCCK0280
BCCK5703
BCCJ0882

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The Peritoneum
Dierega, Gere S.; Rodgers, Kathleen E.;
The Peritoneum, 324-324 (2012)
Yoshikazu Isomura et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(25), 10209-10220 (2013-06-21)
It is widely accepted that dorsal striatum neurons participate in either the direct pathway (expressing dopamine D1 receptors) or the indirect pathway (expressing D2 receptors), controlling voluntary movements in an antagonistically balancing manner. The D1- and D2-expressing neurons are activated
Kelli G Sharp et al.
Experimental neurology, 257, 186-204 (2014-04-22)
As part of the NIH "Facilities of Research Excellence-Spinal Cord Injury" project to support independent replication, we repeated key parts of a study reporting robust engraftment of neural stem cells (NSCs) treated with growth factors after complete spinal cord transection
Krystal Nizar et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(19), 8411-8422 (2013-05-10)
Calcium-dependent release of vasoactive gliotransmitters is widely assumed to trigger vasodilation associated with rapid increases in neuronal activity. Inconsistent with this hypothesis, intact stimulus-induced vasodilation was observed in inositol 1,4,5-triphosphate (IP3) type-2 receptor (R2) knock-out (KO) mice, in which the

Questions

  1. How to use the Dextran Standard 5,000 for glycan conversion (from retention time to GU) in the context of HILIC-HPLC?

    1 answer

    1. Retention times for the various oligosaccharides in this standard will be highly dependent on the specific column used. Dextran is comprised of polymeric glucose, and the partially hydrolyzed mixture can be separated by HILIC according to polarity or by gel-permeation chromatography (GPC) according to size. Various average molecular weight data are reported in the Certificate of Analysis, including the molecular weight for the highest peak (Mp). This information can be used to determine the number of glucose units (GU) represented in each peak. The retention times and GU values for these corresponding peaks then can be used to predict structural information about unknown glycans.

      Conversion of dextran ladder data to determine structural information about structurally complex glycans (such as N-glycans) is more challenging. This often involves using LC-MS data for internal N-glycan calibrants along with an external dextran ladder calibrant to accurately convert retention times into comparable "GU values". Some work has been established in peer-reviewed literature to compile databases for this information, but the relative GU values are highly dependent on the specific glycans being analyzed.

      A general article on the HPLC analysis of glycans, including the use of a dextran ladder, can be found here: https://www.sigmaaldrich.com/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/hplc-analysis-of-glycans

      An article discussing the use of the BIOshell™ Glycan HILIC HPLC Column for glycan analysis, including a procainamide-labeled dextran ladder, can be found here: https://www.sigmaaldrich.com/technical-documents/protocol/analytical-chemistry/large-molecule-hplc/bioshell-glycan-hplc-columns

      The full method used for the HPLC analysis of the procainamide-labeled dextran ladder mentioned above can be found here: https://www.sigmaaldrich.com/technical-documents/chromatograms/hplc/hplc-analysis-of-a-procainamide-labeled-dextran-ladder-on-bioshell-glycan-using-hilic-flr/supelco/g006408?srsltid=AfmBOopcoIvmn2uWXfcXvHYaBb8OnaOjyXALysP-pZqK3wH1qskT7tlr

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