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XNACRL

Sigma-Aldrich

Extract-N-Amp™ Cellular RNA Lysis Buffer

new
greener alternative

Optimized for rapid cell lysis and RNA stabilization

Synonym(s):

RNA extraction buffer, RNA isolation buffer, RNA lysis buffer

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About This Item

UNSPSC Code:
41106306

Quality Level

200

form

liquid

storage condition

OK to freeze (-10 to-25°C)

greener alternative product characteristics

Waste Prevention
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

suitability

suitable for PCR (suitable for RT-qPCR)

greener alternative category

DfS-Developed

shipped in

dry ice

storage temp.

−20°C

General description

The Extract-N-AmpTM Cellular RNA Lysis buffer (XNACRL) is a single working solution that allows for rapid, room-temperature extraction and stabilization of RNA from a range of cells without heat, agitation or clean up. This is a simple and robust method for preparing cell lysate and stabilizing the extracted RNA for gene expression analysis directly from the cell lysate without the need for purification step prior to analysis.

We are committed to bringing you Greener Alternative Products, which belongs to one of the four categories of greener alternatives. This product is a Design for Sustainability (DfS) developed product. Click here to view its DfS scorecard.

Application

  • The Extract-N-AmpTM Cellular RNA Lysis buffer is suitable for direct use with
  • qRT-PCR
  • RT-LAMP
  • NGS library preparation

Features and Benefits

  • From cell lysis to qRT-PCR in 15 minutes
  • High Throughput and automation-friendly
  • No RNA purification or clean up necessary
  • No columns, heat, or centrifugation necessary
  • Compatible with both adherent and non-adherent mammalian cell types
  • Sensitivity range: 10-100k cells •May be paired with DNase I to ensure removal of DNA for more sensitive processes

Physical form

Liquid

Storage and Stability

Freezer (-10 to-25°C)

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Kit Components Only

Product No.
Description
  • Extract-N-AMP Cellular RNA Lysis buffer 5ML, 50ML

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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