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U1875

Sigma-Aldrich

Urease from Canavalia ensiformis (Jack bean)

Type III, glycerol solution, 500-800 units/mL

Synonym(s):

Jack bean urease, Urea amidohydrolase

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About This Item

CAS Number:
9002-13-5
Enzyme Commission number:
3.5.1.5 (BRENDA, IUBMB)
MDL number:
MFCD00070858
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54

biological source

Canavalia ensiformis

type

Type III

form

glycerol solution

mol wt

~544620

concentration

500-800 units/mL

storage temp.

2-8°C

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General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease of jack bean (Canavalia ensiformis), a hexamer of a single chain of 840 amino acid residues, is found in plants, bacteria, fungi, algae, and soil. It is considered the first nickel-containing enzyme and the first enzyme to be crystallized. Ureases exist in several isoforms, some are jack bean urease (JBURE-I), canatoxin (CNTX), and JBURE-IIB. JBURE-I is more abundant than the other two isoforms. Ureases are found in mature seeds.

Application

Urease from Canavalia ensiformis (Jack bean) has been used:
  • to assay creatine and creatine phosphate using the sample extracted from the left ventricle (LV) and septal tissue
  • to determine the concentration of urea in a stabilized urine sample
  • as one of the standards to measure the urease activity of plant extracts

Biochem/physiol Actions

In plants, ureases are essential for the bioavailability/metabolism of nitrogen in nature and defense mechanisms. Ureases are metalloenzymes that catalyze the hydrolysis of urea to release ammonia a nitrogen source for growth and carbon dioxide. They exhibit internal peptides-mediated insecticidal action and fungicidal activity.
Urease activity has been shown to be inhibited by benzpyrene following exposure to light. This inhibition was due to the effect of benzpyrene on urease′s sulfhydryl groups.

Unit Definition

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Fernando Medina Ferrer et al.
Geobiology, 20(1), 79-97 (2021-08-03)
Modern carbonate tufa towers in the alkaline (~pH 9.5) Big Soda Lake (BSL), Nevada, exhibit rapid precipitation rates (exceeding 3 cm/year) and host diverse microbial communities. Geochemical indicators reveal that carbonate precipitation is, in part, promoted by the mixing of calcium-rich
Fernando Medina Ferrer et al.
Microbial biotechnology, 13(6), 1877-1888 (2020-07-29)
Microbial precipitation of calcium carbonate is a widespread environmental phenomenon that has diverse engineering applications, from building and soil restoration to carbon sequestration. Urease-mediated ureolysis and CO2 (de)hydration by carbonic anhydrase (CA) are known for their potential to precipitate carbonate
Melissa Postal et al.
Peptides, 38(1), 22-32 (2012-08-28)
Ureases (EC 3.5.1.5) are metalloenzymes that hydrolyze urea into ammonia and CO(2). These proteins have insecticidal and fungicidal effects not related to their enzymatic activity. The insecticidal activity of urease is mostly dependent on the release of internal peptides after
Effect of light-activated benzpyrene on urease activity.
G C MILLS et al.
Cancer research, 13(1), 69-72 (1953-01-01)
Jitender M Khurana et al.
European journal of medicinal chemistry, 58, 470-477 (2012-11-17)
Novel 12-aryl-8,9,10,12-tetrahydrobenzo[a]xanthene-11-thiones have been synthesized in high yields by treatment of the corresponding oxo analogs with Lawesson's reagent. The structure has been confirmed by X-ray analysis. The compounds were tested for in vitro antifungal activity against Rhizoctonia bataticola, Sclerotium rolfsii
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