SAB4200176

Anti-JNK antibody, Mouse monoclonal

clone 1C2, purified from hybridoma cell culture

• Synonym(s): Anti-JNK-46, Anti-JNK1, Anti-JNK1A2, Anti-JNK21B1/2, Anti-JUN N-terminal kinase, Anti-MAP kinase 8, Anti-MAPK8, Anti-PRKM8, Anti-SAPK1, Anti-stress-activated protein kinase 1
• MDL number: MFCD00802809
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1C2, monoclonal
• mol wt: antigen ~43 kDa
• species reactivity: mouse, rat, human
• concentration: ~1.0 mg/mL
• technique(s): western blot: 1-2 μg/mL using using whole extracts of mouse NIH-3T3 cells
• isotype: IgG2a
• UniProt accession no.: P45983
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MAPK8(5599) ; mouse ... Mapk8(26419) ; rat ... Mapk8(116554)

Description

General description

Anti-JNK antibody, Mouse monoclonal, (mouse IgG2a isotype) is derived from the hybridoma 1C2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a recombinant protein. JNK1 (c-Jun N-terminal kinase), also known as MAPK8 (mitogen-activated protein kinase 8) is a member of the MAP kinase family. This gene is mapped to human chromosome 10q11.

Application

Anti-JNK antibody, Mouse monoclonal has been used in immunoblotting and enzyme linked immunosorbent assay (ELISA).

Biochem/physiol Actions

JNK1 (or MAPK8) is a serine threonine kinase that is a key regulator of cellular apoptosis. During the lack of NFkB stiμLation; persistent activation of JNK1 results in TNF-a induced cell death. However, JNK1 may also function as an apoptotic inhibitor. For instance; JNK1 interacts with WOX1 to inhibit apoptosis. Furthermore, JNK1 phosphorylates Bcl-2 to mediate starvation-induced autophagy, and also activates SIRT1 by phosphorylation during conditions of oxidative stress. JNK1 is activated by UV irradiation and oncoproteins and hence may be implicated in cancer propagation
Monoclonal Anti-JNK antibody is specific for human, mouse and rat JNK1 (43 kDa) and/or JNK2 (55 kDa).

JNK1 is also involved in UV (ultra violet) radiation induced apoptosis, which is thought to be related to cytochrome C-mediated cell death pathway.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable