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NXTRACT

Sigma-Aldrich

NuCLEAR™ Extraction Kit

For mammalian tissue or cultured cells

Synonym(s):

Nuclear Isolation Kit

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About This Item

UNSPSC Code:
41105517
NACRES:
NA.56

Quality Level

300

usage

 kit sufficient for 10 extractions (1 ml packed cell volume)
 kit sufficient for 100 extractions (100 μl packed cell volume)

technique(s)

protein extraction: suitable
western blot: suitable

shipped in

dry ice

storage temp.

−20°C

General description

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.

Application

NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.

Other Notes

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.

Linkage

Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549

Legal Information

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS
  • 3× Dilution and Equilibration Buffer 90 mL
  • P8340Protease Inhibitor Cocktail 1 mLSDS

Pictograms

Corrosion
GHS05

Signal Word

Danger

Hazard Statements

H290,H314

Hazard Classifications

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Storage Class Code

8A - Combustible corrosive hazardous materials

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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F Guidez et al.
Molecular and cellular biology, 18(7), 3851-3861 (1998-06-25)
Granulocyte-macrophage colony-stimulating factor (GM-CSF) and macrophage colony-stimulating factor (M-CSF) independently stimulate the proliferation and differentiation of macrophages from bone marrow progenitor cells. Although the GM-CSF and M-CSF receptors are unrelated, both couple to Ras-dependent signal transduction pathways, suggesting that these
Bela Juhasz et al.
American journal of physiology. Heart and circulatory physiology, 294(3), H1365-H1370 (2008-01-15)
Bromelain (Br), a proteolytic enzyme extracted from the stem of the pineapple, is known to possess anti-inflammatory activity and has been shown to reduce blood viscosity, prevent the aggregation of blood platelets, and improve ischemia-reperfusion (I/R) injury in a skeletal
Sumie Hiramatsu et al.
Scientific reports, 9(1), 3054-3054 (2019-03-01)
Global DNA hypomethylation in CD4+ cells in systemic lupus erythematosus (SLE) was suggested to play a key role in the pathogenesis. To identify new methylation-sensitive genes, we integrated genome-wide DNA methylation and mRNA profiling data in CD4+ cells of MRL/lpr
Wenjing Hao et al.
Redox biology, 18, 43-53 (2018-06-26)
8-Oxoguanine DNA glycosylase 1 (OGG1) initiates the base excision repair pathway by removing one of the most abundant DNA lesions, 8-oxo-7,8-dihydroguanine (8-oxoG). Recent data showed that 8-oxoG not only is a pro-mutagenic genomic base lesion, but also functions as an
K A Lee et al.
Gene analysis techniques, 5(2), 22-31 (1988-03-01)
A convenient and rapid method for preparing soluble extracts from the nuclei of as few as 3 x 10(7) mammalian cells (miniextract procedure) is described. By several criteria, miniextracts are comparable to nuclear extracts prepared from large numbers of cells
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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