M2003

MTT Formazan

powder

• Synonym(s): 1-(4,5-Dimethylthiazol-2-yl)-3,5-diphenylformazan, Thiazolyl blue formazan
• Empirical Formula (Hill Notation): C₁₈H₁₇N₅S
• CAS Number: 57360-69-7
• Molecular Weight: 335.43
• NACRES: NA.47
• PubChem Substance ID: 24896591
• UNSPSC Code: 12171500
• MDL number: MFCD00080731

Properties

• assay: ≥97% (TLC)
• Quality Level: 200
• form: powder
• color: purple
• solubility: acetone: 1 mg/mL
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
• InChI: 1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
• InChI key: GIFKVQOPAJDJLO-SZVLODPRSA-N

Description

Application

MTT Formazan has been used to measure cell viability.

Biochem/physiol Actions

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)