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Key Documents

CCF100

Sigma-Aldrich

CM Sepharose

Fast Flow

Synonym(s):

Carboxymethyl Sepharose

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
47101511
NACRES:
NA.56

Quality Level

form

suspension

technique(s)

affinity chromatography: suitable

matrix

6% cross-linked agarose

particle size

45-165 μm (wet bead)

pore size

4,000,000 exclusion limit (av. mol. wt.)

pH

4—13

capacity

90-130 μeq/mL(gel basis)

storage temp.

2-8°C

General description

Preswollen in 20% ethanol
CCF100-500ml′s updated product number is GE17-0719-01
25 ml is GE17-0719-10

Application

CM Sepharose is used in affinity chromatography, protein chromatography and ion exchange chromatography. CM Sepharose hase been used to study diet supplements and to characterize biochemical and enzymatic properties of Viperidae venom.

Legal Information

Sepharose is a trademark of Cytiva

replaced by

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

100.4 - 109.4 °F

Flash Point(C)

38 - 43 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biochemical and enzymatic characterization of BpMP-I, a fibrinogenolytic metalloproteinase isolated from Bothropoides pauloensis snake venom.
Naves de Souza, D.L., et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 161, 102-109 (2012)
Balsamin, a novel ribosome-inactivating protein from the seeds of Balsam apple Momordica balsamina.
Kaur, I., et al.
Amino Acids (2011)
Fei Wang et al.
Methods (San Diego, Calif.), 53(3), 208-213 (2010-12-24)
The infectivity associated with prion disease sets it apart from a large group of late-onset neurodegenerative disorders that shares the characteristics of protein aggregation and neurodegeneration. The unconventional infectious agent, PrP(Sc), is an aberrantly folded form of the normal prion
Meifang Xiao et al.
Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 36(11), 1065-1070 (2011-12-16)
To purify and identify HMGB1 secreted by liver cells HepG2 and immune cells U937. We cultured the liver cell lines HepG2 and immune cell lines U937, and stimulated them with HMGB1 (400 ng/mL) for 20 h. Then the supernatant was
Ajith Anand et al.
Molecular plant-microbe interactions : MPMI, 17(12), 1306-1317 (2004-12-16)
A transgenic wheat line constitutively expressing genes encoding a class IV acidic chitinase and an acidic beta-1,3-glucanase, showed significant delay in spread of Fusarium head blight (scab) disease under greenhouse conditions. In an earlier work, we observed a lesion-mimic phenotype

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