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Key Documents

KYSE-410

94072023, human esophagus, Epitheloid

Synonym(s):

KYSE0410 Cells, KYSE410 Cells

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About This Item

UNSPSC Code:
41106514

product name

KYSE-410, 94072023

biological source

human esophagus

growth mode

Adherent

karyotype

Hypotetraploid with 4% polyploidy

morphology

Epitheloid

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

Cell Line Origin

Human oesophageal squamous cell carcinoma

Cell Line Description

Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18

Culture Medium

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)

Subculture Routine

Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4x10,000 cells/cm2 using trypsin/EDTA;5% CO2; 37°C. Doubling time ~45h

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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