C2037
Cytochrome c from bovine heart
≥95% based on Mol. Wt. 12,327 basis
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About This Item
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biological source
bovine heart
Assay
≥95% based on Mol. Wt. 12,327 basis
form
powder or crystals
mol wt
12327 Da
technique(s)
cell based assay: suitable
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
storage temp.
−20°C
Gene Information
cow ... CYC1(512500)
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General description
Cytochrome C is a heme protein. It is localized between the inner and outer mitochondrial membranes.
Application
Cytochrome c from bovine heart has been used:
- for cytochrome oxidase staining using the mice brain tissue
- as a component of control mixture to evaluate the accuracy of tandem mass spectra in identifying peptides
- to study its effect on the stability, transport, and retention of biochar colloids (BCs) in saturated porous media under various solution pH and ionic strength (IS) conditions
- as a component of staining solution for the detection of cytochrome oxidase activity in the brain tissue sections of mouse
- as a component in the assay to study its superoxide scavenging efficiency
- as a component of serum-free DMEM media to evaluate non-specific cytochrome C reduction in astrocytes and HT-29 cells
Biochem/physiol Actions
Cytochrome C is a key mediator of oxidative phosphorylation. It mediates the electron transport between complex III and complex IV of the respiratory chain.
Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome.
Cytochrome c has been identified as an important mediator in apoptotic pathways. The release of mitochondrial cytochrome c into the cytoplasm stimulates apoptosis and is commonly used as an indicator of the apoptotic process in the cell. Investigation on the effect of Paris Saponin I (PS I) on human gastric carcinoma cell growth (SGC7901 cells) have shown an elevated level cytoplasmic cytochrome c. Results are an inhibition of proliferation in SGC7901 cells by inducing mitochondria-dependent apoptosis through cytochrome.
The ready interconversion of cytochrome c between ferrous and ferric states makes it an efficient biological electron carrier. It plays a vital role in cellular oxidations in both plants and animals. Generally regarded as a universal link in the respiratory chain, it forms the essential electron-bridge between the respirable substrates and oxygen.
Preparation Note
Prepared using TCA.
Other Notes
View more information on cytochrome c and electron transport at www.sigma-aldrich.com/enzymeexplorer.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Chromatograms
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