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A9207

Sigma-Aldrich

Avidin−Agarose from egg white

aqueous glycerol suspension

Synonym(s):

Avidin agarose

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About This Item

MDL number:
MFCD00130572
UNSPSC Code:
41106514
NACRES:
NA.56

biological source

egg white

conjugate

agarose conjugate

form

aqueous glycerol suspension

matrix

cross-linked 6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

amino

matrix spacer

15 atoms

storage temp.

−20°C

Related Categories

General description

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

Application

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Avidin-Agarose from egg white has been used:
  • in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
  • in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
  • in avidin bead assay for purification of labelled biotin
  • for capture of glycoproteins from murine cornea samples

Biochem/physiol Actions

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

Unit Definition

One unit will bind 1.0 μg of d-biotin.

Physical form

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Senarath Dissanayake
Molecular immunology, 38(12-13), 911-919 (2002-05-16)
Fucosylated sugars in Schistosoma mansoni possess immunomodulatory properties. In order to gain insights to the mechanisms involved, attempts were made to identify host immune cell molecules that specifically recognize these sugars. On Western blots, specific binding of synthetic biotinylated fucose
Corneal surface glycosylation is modulated by IL-1R and Pseudomonas aeruginosa challenge but is insufficient for inhibiting bacterial binding
Jolly AL, et al.
Faseb Journal, 31(6), 2393-2404 (2017)
Abelardo Medina et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 17(2), 268-277 (2009-03-27)
Transdifferentiation is a process in which the original commitment of a cell is changed to give rise to unexpected peripheral mature cells. Our previous report showed that circulating stem cells can generate keratinocyte-like cells (KLCs). However, it remains to be
Jens Spross et al.
Analytical and bioanalytical chemistry, 402(7), 2395-2405 (2012-01-21)
A poly(glycidyl methacrylate-co-acrylamide-co-ethylene dimethacrylate) monolith and a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique. The biotin binding capacity of monolithic affinity columns with immobilized monomeric
D Scott Wilbur et al.
Bioconjugate chemistry, 21(7), 1225-1238 (2010-07-06)
Previous studies have shown that pretargeting protocols, using cancer-targeting fusion proteins, composed of 4 anti-CD20 single chain Fv (scFv) fragments and streptavidin (scFv(4)-SAv), followed by a biotinylated dendrimeric N-acetyl-galactosamine blood clearing agent (CA), 1, then a radiolabeled DOTA-biotin derivative (a
View All Related Papers

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Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

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