This α-Amylase is sold based on mass, and is guaranteed to have a specific activity that is equal to or greater than 1000U/mg. Therefore, the 10mg size will have a minimum of 10,000 units.
The lot-specific Certificate of Analysis will provide the assayed specific activity:
https://www.sigmaaldrich.com/product/sigma/a6255#product-documentation
A6255
α-Amylase from porcine pancreas
PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)
Synonym(s):
β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase
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About This Item
EC Number:
MDL number:
UNSPSC Code:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Pricing and availability is not currently available.
Recommended Products
biological source
Porcine pancreas
Quality Level
type
Type I-A
form
saline suspension
specific activity
≥1000 units/mg protein (E1%/280)
mol wt
51-54 kDa
greener alternative product characteristics
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Design for Energy Efficiency
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shipped in
wet ice
storage temp.
2-8°C
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General description
Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.
Application
α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A6255 is from porcine pancreas and is type I-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis [1].
Biochem/physiol Actions
α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability.
Unit Definition
One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.
Physical form
Suspension in 2.9 M NaCl solution containing 3 mM CaCl2
Preparation Note
PMSF treated. 2× crystallized
Other Notes
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
inhibitor
Product No.
Description
Pricing
substrate
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Certificates of Analysis (COA)
Lot/Batch Number
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Protocols
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.
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A 6255 is declared with an activity of 1000 U/mg. 10 mg of enzyme contain 10000 units or not? What is the amount of liquid in the pack?
1 answer-
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I have A6255 enzyme of 10 mg. If I dissolve it with 1 ml of buffer, it means that the activity is about 10000 U/ml. What I mean is that it contains 10 mg of solid or 10 mg of protein?
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The activity of this product, ?-Amylase from porcine pancreas, is batch specific. An example batch 0000367139 has 24 mg protein/ml (UV) and 1162 units/mg protein. Therefore, a 10 mg vial from this batch would have 11,620 units. For more information, refer to the following documentation: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A6255-10MG0000367139.pdf
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What is the solution stablity of Product No. A6255, α-Amylase from porcine pancreas?
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α-Amylase is stable in 25 mM Tris-HCl, pH 7.5, with 100 mM KCl, refrigerated at near 0 °C or frozen at -20 °C for at least 9 days. Another recommended storage condition is in 1 mM phosphate, pH 7.3, with 30 mM CaCl2 at -15 °C.
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How should Product No. A6255, α-Amylase from porcine pancreas, be dissolved?
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The product is soluble in water (1 mg/mL), yielding a clear, colorless solution.
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How is Product No. A6255, α-Amylase from porcine pancreas, used?
1 answer-
α-Amylase is an enzyme that breaks down polysaccharides, such as starch and glycogen, to give glucose and maltose. α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. The α-(1,6) bond is not hydrolyzed.
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How does the storage temperature relate to shipping conditions?
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The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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What is the recommended substrate for the α-Amylase test of Product No. A6255?
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The recommended substrate for the enzymatic α-Amylase test is Product No. S2630, Starch.
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