A0157
Ascorbate Oxidase from Cucurbita sp.
lyophilized powder, 1,000-3,000 units/mg protein
Synonym(s):
L-Ascorbate:oxygen oxidoreductase, Ascorbase
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About This Item
Recommended Products
biological source
plant (Cucurbita spp.)
Quality Level
form
lyophilized powder
specific activity
1,000-3,000 units/mg protein
storage temp.
2-8°C
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General description
Ascorbate oxidase is a homodimeric enzyme, which comprises 552 amino acid residues per subunit (zucchini). It corresponds to a molecular mass of 70kDa per subunit. This enzyme is mainly found in plants, fungi and eubacteria.
Application
Ascorbate Oxidase from Cucurbita sp. has been used:
- as a supplement in the culture medium for differentiation into osteoblasts
- to oxidize ascorbic acid producing monodehydroascorbate in monodehydroascorbate reductase assay
- in determining ascorbate (AsA) and dehydroascorbate (DHA) concentrations
Ascorbate oxidase, from Cucurbita sp., may be used to study oxidative stress and heat stress response and tolerance. Ascorbate oxidase, from Sigma, has been used in ascorbic acid assays to study the heat stress response of Arabidopsis .
Biochem/physiol Actions
Ascorbate oxidase (AO) participates in cell growth by regulating reduction/oxidation (redox) of the apoplast. This enzyme can synthesize the oxidative molecule dehydroascorbate acid (DHA) in the apoplast. It is also involved in cell elongation and enlargement development.
Ascorbate oxidase converts ascorbic acid to dehydroascorbic acid. It is highly specific for L-ascorbic acid and a few analogs. Ascorbate oxidase exists as a dimer and has a molecular weight of approximately 140 kDa.
Unit Definition
One unit will oxidize 1.0 μmole of L-ascorbate to dehydroascorbate per min at pH 5.6 at 25 °C.
Physical form
Lyophilized powder containing buffers and sucrose as stabilizer.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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Cécile Garchery et al.
Plant, cell & environment, 36(1), 159-175 (2012-06-26)
The regulation of carbon allocation between photosynthetic source leaves and sink tissues in response to stress is an important factor controlling plant yield. Ascorbate oxidase is an apoplastic enzyme, which controls the redox state of the apoplastic ascorbate pool. RNA
Irina I Panchuk et al.
Plant physiology, 129(2), 838-853 (2002-06-18)
To find evidence for a connection between heat stress response, oxidative stress, and common stress tolerance, we studied the effects of elevated growth temperatures and heat stress on the activity and expression of ascorbate peroxidase (APX). We compared wild-type Arabidopsis
Ethylene response factor 6 is a regulator of reactive oxygen species signaling in Arabidopsis
Sewelam N, et al.
PLoS ONE, 8(8), e70289-e70289 (2013)
Direct electrochemistry of proteins and enzymes
Perspectives in bioanalysis, 1, 517-598 (2005)
A Messerschmidt et al.
Journal of molecular biology, 224(1), 179-205 (1992-03-05)
The crystal structure of the fully oxidized form of ascorbate oxidase (EC 1.10.3.3) from Zucchini has been refined at 1.90 A (1 A = 0.1 nm) resolution, using an energy-restrained least-squares refinement procedure. The refined model, which includes 8764 protein
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