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W0625

Sigma-Aldrich

Wright stain

≤4% purity (HPLC), powder

Synonym(s):

Eosin Methylene blue according to Wright

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12171500
NACRES:
NA.47

product name

Wright stain, suitable for blood stain

Assay

≤4% (HPLC)

Quality Level

form

powder

concentration

≤4% (HPLC)

color

dark green

solubility

methanol: 10 mg/10 mL

suitability

suitable for blood stain

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1

InChI key

AXIKDPDWFVPGOD-UHFFFAOYSA-O

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General description

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Application

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.
  • It is also used for G-banding and helps in the analysis of poorly spread metaphases.
  • It has been used to study the cytogenetics of human hematopoietic cells.
  • It has also been employed in the karyotyping of human embryonic stem cells.
  • It is also used in the staining of chromosomes.
  • It is used for the exudate analysis of white blood cell concentration.
Reported to be superior to May-Grunwald Stain in blood staining.

Features and Benefits

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Preparation Note

Prepared using polychromed (oxidized) methylene blue and eosin Y.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Bernadette F. Rodak, George A. Fritsma, Kathryn Doig
Hematology: Clinical Principles and Applications (2007)
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Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia
Kim, et al.
Cell, 173 (2018)
Lillie, R.D.
Conn?s Biological Stains, 495-495 (1977)
Liangqun Xie et al.
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