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MABT163

Sigma-Aldrich

Anti-Gamma-1-Tubulin, clone 6H3.1 Antibody

clone 6H3.1, from mouse

Synonym(s):

Tubulin gamma-1 chain, Gamma-1-tubulin, Gamma-tubulin complex component 1, GCP-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6H3.1, monoclonal

species reactivity

human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_001061

UniProt accession no.

P23258

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TUBG1(7283)

General description

Gamma-1-Tubulin, also known as Gamma-tubulin complex component 1, GCP-1 or Tubulin gamma-1 chain, and encoded by the gene TUBG1/TUBG, is a form of tubulin protein and a component of microtubules. Tubulin is the major constituent of microtubules. The gamma chain is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome and plays a major role in the organization of the microtubule cytoskeleton. Gamma-1-Tubulin is part of the pericentriolar matrix component that regulates alpha/beta chain minus-end nucleation, and it is also involved in centrosome duplication and spindle formation. Interestingly, gamma tubulin is often up regulated but improperly compartmentalized in many cancer cells including non-small cell lung cancer. Mutations in Gamma-1-Tubulin gene are associated with developmental cortical defects including microcephaly and malformations of cortical development (MCD). Gamma-1-Tubulin is localized to the cytoplasm and cytoskeleton components and expressed in a variety of cell types and tissues.

Immunogen

GST-tagged recombinant protein corresponding to human Gamma-1-Tubulin.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Gamma-1-Tubulin in NIH/3T3 cells.

Alexa Fluor™ is a registered trademark of Life Technologies.
This Anti-Gamma-1-Tubulin antibody, clone 6H3.1 is validated for use in western blotting & ICC for the detection of Gamma-1-Tubulin.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Gamma-1-Tubulin in 10 µg of HeLa cell lysate.

Target description

~56 kDa observed. Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jens S Andersen et al.
Nature communications, 15(1), 2687-2687 (2024-03-28)
Centrosomes and cilia are microtubule-based superstructures vital for cell division, signaling, and motility. The once thought hollow lumen of their microtubule core structures was recently found to hold a rich meshwork of microtubule inner proteins (MIPs). To address the outstanding

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