MABT121

Anti-Vimentin Antibody, clone 3CB2

clone 3CB2, from mouse

• Synonym(s): Vimentin
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3CB2, monoclonal
• species reactivity: chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard
• species reactivity (predicted by homology): chicken (immunogen homology)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgMκ
• NCBI accession no.: NP_001041541
• UniProt accession no.: P09654
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... VIM(7431)

Description

General description

Vimentin is a class III intermediate filament protein (homopolymers) found in various non-epithelial cells including mesenchymal cells and fibroblasts. In many cells of mesenchymal origin, it is one of the most prominent phosphoproteins, especially during cell division when enhanced phosphorylation of vimentin corresponds with the reorganization of vimentin filaments.

Immunogen

Vimentin extracted from chicken embryonic brain tissue.

Application

Anti-Vimentin Antibody, clone 3CB2 is an antibody against Vimentin for use in WB, IC, IH.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human central and peripheral retinal sections (Perez-Alvarez, M.J., et al. (2008). J Neurosci Res. 86(8):1871-1883).

Western Blot Analysis:
A representative lot was used to detect vimentin in chicken and rat total brain extracts (Prada, FA., et al. (1995). GLIA 15:389-400).

Immunohistochemistry Analysis:
A representative lot was used to detect vimentin in E3 chick embryo sections and different regions of the developing chick central nervous system (Prada, FA., et al. (1995). GLIA 15:389-400).

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blot in ARPE19 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Vimentin in ARPE19 cell lysate.

Target description

~54 kDa observed

Physical form

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
ARPE cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable