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biological source
human
Quality Level
Assay
>98% (SDS-PAGE)
form
liquid
manufacturer/tradename
Chemicon®
shipped in
wet ice
General description
DENSITY:
0.95-1.006 g/mL
0.95-1.006 g/mL
Product Source: Human plasma tested negative for HBsAg and HIV-I, HIV-II, HBc and Hepatitis C antibodies. All blood products should be treated as potentially infectious.
VLDL is isolated from fresh human plasma containing EDTA and sodium citrate by sequential isopycnic ultracentrifugation at the lower and upper density limits of the specified density range (Havel et al., 1955). Density adjustments are performed with solid KBr. VLDL is washed by centrifugation at the upper density limit to ensure removal of higher density proteins.
Quality
QUALITY ASSURANCE:
Protein determination by the Pierce BCA method using 1% SDS with BSA as the standard. Purity of each preparation is verified by agarose gel electrophoresis using Fat red 7B staining for lipid (2,3).
Protein determination by the Pierce BCA method using 1% SDS with BSA as the standard. Purity of each preparation is verified by agarose gel electrophoresis using Fat red 7B staining for lipid (2,3).
Physical form
Liquid in 150 mM NaCl, pH 7.4 with 0.01% EDTA. Filtered to sterility using 0.2 micron filter in a sterile vial.
COMPOSITION:
88-95% lipid, 5-12% protein
COMPOSITION:
88-95% lipid, 5-12% protein
Storage and Stability
Maintain at 2-8°C for up to four weeks. DO NOT FREEZE. Freezing may cause denaturation.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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