Our laboratory has not specifically studied the impact of high salt, such as 500 mM NaCl, on the activity of RNAseA. However, Park and Raines (2003) have investigated the activity of RNAseA under high salt concentration, up to 1 M NaCl. The results available in the publication titled "Catalysis by ribonuclease A is limited by the rate of substrate association" may serve as a useful reference. The study is available in Biochemistry, 2003 Apr 1;42(12):3509-18. From the study, it is noted that "the pKa values at 1.0 M NaCl are expected to be higher by 0.4-0.5 unit than the pKa values determined at 0.142 M NaCl."
556746
RNase A, Protease-Free, Highly Purified, Bovine Pancreas
For selective degradation of RNA
Synonym(s):
RNase A, Protease-Free, Highly Purified, Bovine Pancreas, Ribonuclease A
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All Photos(1)
About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
Pricing and availability is not currently available.
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Quality Level
Assay
≥95% (SDS-PAGE)
form
lyophilized solid
specific activity
81-99 units/mg material
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated
solubility
water: 2 mg/mL
foreign activity
Protease, none detected
shipped in
ambient
storage temp.
−20°C
SMILES string
[nH]1cnc(c1)CC(NC(=O)CCN)C(=O)O
InChI
1S/C9H14N4O3/c10-2-1-8(14)13-7(9(15)16)3-6-4-11-5-12-6/h4-5,7H,1-3,10H2,(H,11,12)(H,13,14)(H,15,16)
InChI key
CQOVPNPJLQNMDC-UHFFFAOYSA-N
General description
Note: 1 KU = 1000 units.
Ribonuclease A, Protease-Free is a
chromatographically purified, pyrimidine-specific
endoribonuclease that acts on single-stranded
RNA.
chromatographically purified, pyrimidine-specific
endoribonuclease that acts on single-stranded
RNA.
Ribonuclease A, Protease-Free is achromatographically purified, pyrimidine-specificendoribonuclease that acts on single-strandedRNA.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will catalyze the hydrolysis of RNA to yield a first-order velocity constant equal to 1 at 25°C, pH 5.0.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
Analysis Note
May contain trace amounts of DNase I, which can be inactivated by boiling for 15 min in 50 mM potassium acetate, pH 5.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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