05-1308
Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3, rabbit monoclonal
clone Apu3, from rabbit
Synonym(s):
Anti-Ubiquitin, Clone Apu3 Anti-Ubiquitin, Lys63-Specific Ubiquitin Antibody
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All Photos(7)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
Apu3, monoclonal
species reactivity
rat, human, mouse, rabbit
technique(s)
cell based assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... UBAP2(55833)
General description
DI-UBIQUITIN PROTEIN AVAILABLE!
Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388)
Di-Ubiquitin (Lys63-linked) Recombinant Protein (17-10388)
Polyubiquitin chains linked through the Lys63 residue of ubiquitin (Ub) are associated with nonproteolytic functions such as signal transduction. Lys63-linked polyUb chains connect components of NFκB signaling in a highly regulated manner, and genetic evidence indicates the involvement of Lys63-linked Ub chains in stress response and DNA repair. Research in yeast has suggested Lys63-linked Ub chains stimulate endocytosis.
In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
In contrast, polyubiquitin chains linked through the Lys48 residue of ubiquitin are most commonly associated with proteins targeted for proteosomal degradation.
Specificity
Based on sequence homology, broad species cross-reactivity is expected.
Recognizes ubiquitin linked at Lys63. Does not recognize ubiquitin linked at Lys48 or free ubiquitin.
Immunogen
Epitope: Lys63 ubiquitination site
Immunogen used was human di-ubiquitin linked at Lys63.
Application
Immmunoprecipitation:
Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysates
Immunohistochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells.
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red).
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Immunocytochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections.
Rabbit monoclonal anti-ubiquitin (Lys-63-specific), clone Apu3, precipitated ubiquitin-containing proteins from HeLa cell lysates
Immunohistochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells.
Flow cytometry:
Flow cytometry analysis of Jurkat cells using Anti-ubiquitin Lys63-specific (#05-1308) (blue) and rabbit IgG control (red).
Inhibition Assay:
Preincubation of anti-ubiquitin, Lys63-specific, with K63-multiubiquitin chains inhibited binding to HeLa cell lysate in Western blots.
Immunocytochemistry:
Ubiquitin (Lys63-specific) staining of colorectal cancer tissue sections is restricted to tumor cells. Positive staining was also detected in mouse, rat, and rabbit brain sections.
Anti-Ubiquitin Antibody, Lys63-Specific, clone Apu3 is a high quality Rabbit Monoclonal Antibody for the detection of Ubiquitin and has been validated in Inhibition Assay (PIA), Immunocytochemistry (ICC), Immunoprecipitation (IP), Immunohistochemistry (IHC) and Western Blotting (WB).
Research Category
Signaling
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
Ubiquitin & Ubiquitin Metabolism
Quality
Western Blot Analysis:
A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains.
A 1:1000 dilution of this antibody detected Lys-63 ubiquitin chains by Western blotting, but did not detect free ubiquitin or Lys48 polyubiquitin chains.
Target description
varies
Physical form
Format: Purified
Protein A purified
Protein A purified recombinant rabbit monoclonal antibody that consists of a humanized fAb fragment fused to a rabbit Fc tail produced in a CHO recombinant antibody system. Supplied in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl, 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Analysis Note
Control
Lys63 polyubiquitin chains.
Lys63 polyubiquitin chains.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Gustavo M Silva et al.
Nature structural & molecular biology, 22(2), 116-123 (2015-01-27)
Ubiquitination is a post-translational modification that signals multiple processes, including protein degradation, trafficking and DNA repair. Polyubiquitin accumulates globally during the oxidative stress response, and this has been mainly attributed to increased ubiquitin conjugation and perturbations in protein degradation. Here
Atypical ubiquitin chains: new molecular signals. 'Protein Modifications: Beyond the Usual Suspects' review series.
Ikeda, Fumiyo and Dikic, Ivan
EMBO Reports, 9, 536-542 (2008)
The RIDDLE syndrome protein mediates a ubiquitin-dependent signaling cascade at sites of DNA damage.
Grant S Stewart et al.
Cell, 136(3), 420-434 (2009-02-11)
The biological response to DNA double-strand breaks acts to preserve genome integrity. Individuals bearing inactivating mutations in components of this response exhibit clinical symptoms that include cellular radiosensitivity, immunodeficiency, and cancer predisposition. The archetype for such disorders is Ataxia-Telangiectasia caused
Keiko Taguchi et al.
Proceedings of the National Academy of Sciences of the United States of America, 109(34), 13561-13566 (2012-08-09)
The Kelch-like ECH-associated protein 1 (Keap1)-NF-E2-related factor 2 (Nrf2) system is essential for cytoprotection against oxidative and electrophilic insults. Under unstressed conditions, Keap1 serves as an adaptor for ubiquitin E3 ligase and promotes proteasomal degradation of Nrf2, but Nrf2 is
Unique insights into maternal mitochondrial inheritance in mice.
Luo, SM; Ge, ZJ; Wang, ZW; Jiang, ZZ; Wang, ZB; Ouyang, YC; Hou, Y; Schatten, H; Sun, QY
Proceedings of the National Academy of Sciences of the USA null
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