A spectrophotometric assay for feruloyl esterases.

A spectrophotometric assay for feruloyl esterases.

Analytical biochemistry (2002-10-17)

Vladimír Mastihuba, Lubomír Kremnický, Mária Mastihubová, J L Willett, Gregory L Côté

ABSTRACT

We have developed a spectrophotometric assay for the quantitative determination of feruloyl esterase activity based on release of 4-nitrophenol from a novel substrate, 4-nitrophenyl ferulate in an emulsion of Triton X-100 in aqueous buffer solution. The release of 4-nitrophenol was linear with reaction time at an early stage of the reaction with various esterase preparations. The method proposed here is accurate, rapid, and easy to perform.

MATERIALI

N° Catalogo

Marchio

Descrizione del prodotto

61716

Sigma-Aldrich

4-Nitrophenyl dodecanoate, ≥98.0% (GC)