RNA from SARS-CoV-2, Influenza A and B, and Respiratory Syncytial virus (RSV) have been detected using VRE100 to lyse crosslink-inactivated viral particles in Viral Transport Medium.
VRE100
Viral RNA Extraction Buffer
One Step RT-qPCR Compatible, Optimized for Viral RNA Detection in Saliva
Sinonimo/i:
For use with Reverse Transcription-qPCR, Viral Lysis Solution for RT-qPCR, Viral RNA Extraction
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0.5 ML
CHF 125.00
5 ML
CHF 917.00
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Cambia visualizzazione
0.5 ML
CHF 125.00
5 ML
CHF 917.00
About This Item
Codice UNSPSC:
12161703
NACRES:
NA.52
Prodotti consigliati
Grado
Molecular Biology
Livello qualitativo
Stato
liquid
Concentrazione
4X
tecniche
LAMP: suitable
PCR: suitable
RT-PCR: suitable
qPCR: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
−20°C
Descrizione generale
The Viral RNA Extraction Buffer has been optimized to provide rapid, room-temperature lysis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral particles in saliva. The buffer comes as a 4x working solution that can be mixed with virus-containing samples in a qRT-PCR plate. Upon 5 minute incubation at room temperature, viral particles are lysed and RNA is stabilized such that samples are ready for direct analysis via RT-qPCR with no additional cleanup step. Removal of precipitates in saliva via centrifugation prior to testing is recommended for optimal sensitivity.
Applicazioni
The Viral RNA Extraction Buffer is broadly compatible with most nucleic acid detection methods and does not reduce signal when used at working concentration in qRT-PCR. qRT-PCR compatibility has been demonstrated in a 4-fluor multiplex setting consisting of Cy5, TxRed, FAM, and HEX.
RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.
RT-qPCR compatibility has been verified with several one-step RT-qPCR kits:
- Quantitative RT-PCR ReadyMix™ (Sigma-Aldrich, catalog QR0200)
- KAPA PROBE FAST One-Step Universal (Sigma-Aldrich, catalog KK4752)
- KiCqStart® One-Step Probe RT-qPCR ReadyMix™ (Sigma-Aldrich, catalog KCQS07)
- TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher, catalog A28525)
The Viral RNA Extraction Buffer is also compatible with colorimetric LAMP assays.
Caratteristiche e vantaggi
- Extracted viral RNA can be used for detection without cleanup
- Directly compatible with quantitative reverse transcription polymerase chain reaction (qRT-PCR) and loop mediated isothermal amplification(LAMP) assays
- Ideal for enveloped RNA viruses in saliva, saline, Amies medium, and viral transport medium
- Highly sensitive and faster than other leading methods, lysis by Proteinase K and heating
Note legali
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Eye Dam. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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Articoli
Viral RNA Extraction Buffer (VRE100) and related reagents for qRT-PCR and additional viral RNA analyses.
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Which viruses can be detected using VRE100?
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What ratio of VRE100 to sample is recommended?
1 answer-
VRE100 was developed as a 4x lysis solution, such that 1 part buffer is added to 3 volume equivalents of sample. However, improved detection sensitivity may be achieved in certain samples by increasing the amount of VRE100 used for sample lysis.
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Can samples lysed with VRE100 be stored for future use?
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Yes, treated samples can be stored at -20 °C until ready for RNA detection. Multiple freeze-thaws are not recommended.
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Which viral transport media can be used with VRE100?
1 answer-
VRE100 has been shown to be compatible with saline, including phosphate-buffered saline (PBS) and Hank’s Buffered Saline Solution (HBSS), as well as with standard viral transport medium containing 2% FBS. Transport media containing guanidine are not recommended for use with VRE100 as guanidine will interfere with direct nucleic acid detection techniques, such as RT-qPCR. VRE100 is also compatible with undiluted saliva.
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Is VRE100 capable of inactivating virus?
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Viral inactivation after incubation with VRE100 has not been tested.
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Is there any reaction stoppage step required?
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No reaction stoppage step is required. Treated samples can be kept at room temperature for up to 30 min. If analyzing RNA within 1-2 hours samples can be kept on ice, otherwise samples should be frozen until ready for RNA detection.
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Can VRE100 be used for nasal swabs?
1 answer-
VRE100 has not been tested for use with nasal swabs, and is a Research Use Only product.
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