V7259

Vascular Endothelial Growth Factor human

VEGF, recombinant, expressed in E. coli, powder, suitable for cell culture

• Sinonimo/i: VEGF
• Numero CAS: 127464-60-2
• Numero MDL: MFCD00286542
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Origine biologica: human
• Livello qualitativo: 200
• Ricombinante: expressed in E. coli
• Saggio: ≥98% (SDS-PAGE)
• Forma fisica: powder
• Potenza: 1-10 ng/mL ED₅₀/EC₅₀
• Qualità: endotoxin tested
• PM: protein 38.2 kDa (as homodimer)
• Confezionamento: pkg of 10 μg
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: ≤0.1 EU/μg
• Colore: white
• Solubilità: water: soluble
• N° accesso UniProt: P15692
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... VEGFA(7422)

Descrizione

Descrizione generale

Vascular Endothelial Growth Factor (VEGF) gene is localized to human chromosome 6p21.3, and is thought to encode four different isoforms.

Applicazioni

Vascular Endothelial Growth Factor (VEGF) has been used for-

• the determination of oligodendrocyte precursor cells (OPCs) and luteal cell proliferation/survival
• inducing proliferation of epithelial cell clusters (ECCs) to be transplanted into mice

Azioni biochim/fisiol

Vascular Endothelial Growth Factor (VEGF), also known as vasculotropin, is an angiogenic growth factor, which is heat and acid stable. VEGFs stimulate endothelial cell growth, angiogenisis, and capillary permeability. VEGF is a secreted homodimeric, heparin-binding glycoprotein,1 which has an isoelectric point of 8.5. VEGF promotes the growth of endothelial cells isolated from bovine adrenal cortex, cerebral cortex, fetal and adult aorta, and human umbilical vein. The target cell specificity of VEGF is restricted to vascular endothelial cells. VEGF has no mitogenic effect on cultured corneal endothelial cells, vascular smooth muscle cells, BHK-12 fibroblasts, keratinocytes, human sarcoma cells, or lens epithelial cells. A variety of human tumor cell lines including sarcoma and carcinoma cells show a 3.7 kb RNA transcript that hybridizes with the VEGF probe in a Northern blot. Mouse sarcoma 180 cells express the VEGF mRNA and secrete a VEGF-like mitogen.

VEGF recombinant protein might have potential as a therapeutic agent in treatment-resistant disorders, where it can facilitate endothelial cell growth and vessel formation.

Proprietà fisiche

Monomer consists of 165 amino acid residues, approx. mol. weight 19.2 kDa

Stato fisico

Lyophilized from a sterile filtered buffered solution with no additives.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)