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T8154

Trypan Blue

0.4%, liquid, sterile-filtered, suitable for cell culture

Sinonimo/i:

Blue cell viability dye

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Informazioni su questo articolo

Formula empirica (notazione di Hill):
C34H24N6Na4O14S4
Numero CAS:
72-57-1
Peso molecolare:
960.81
UNSPSC Code:
12352207
NACRES:
NA.75
PubChem Substance ID:
57654704
MDL number:
MFCD00003969
Beilstein/REAXYS Number:
4360496
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sterility

sterile-filtered

Quality Segment

200

form

liquid

storage condition

dry at room temperature

concentration

0.4%

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

application(s)

cell analysis

shipped in

ambient

storage temp.

room temp

SMILES string

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4

InChI key

GLNADSQYFUSGOU-UHFFFAOYSA-J

General description

Il medesimo prodotto è disponibile anche in un pratico formato liofilizzato (polvere); si rimanda, a tal proposito, al codice T6146.

Il Trypan blue (TB), un colorante azoico anionico idrofilo, attraversa la membrana cellulare soltanto delle cellule morte, quindi permette di evidenziare in blu le cellule/i tessuti morti. È utilizzato in lavori di microscopia quantitativa per la tecnica di colorazione per esclusione, che differenzia tra le cellule di mammifero vive e quelle morte.[1]
Il trypan blue è un colorante usato per differenziare le cellule vive da quelle morte. È un colorante vitale che non viene assorbito dalle cellule vitali sane, ma che colora quelle con membrana cellulare danneggiata. In questo modo è possibile effettuare il conteggio delle sole cellule morte. A volte il metodo viene definito metodo di colorazione per esclusione.

Application

La soluzione di Trypan blue è stata usata:
  • nel saggio di vitalità cellulare per il conteggio delle cellule vive e di quelle morte;[2]
  • nel saggio di salvataggio ("rescue") dall'apoptosi per il conteggio delle cellule vive;[2]
  • nel metodo di conteggio per esclusione per determinare la curva di crescita;[3]
  • come controllo in soluzione salina e nella preparazione di una soluzione con lipopolissacaridi (LPS) per confermare la buona riuscita dell'infusione di LPS;[4]
  • nel test di esclusione con colorante trypan blue per determinare la vitalità delle cellule epiteliali gastriche (GEC) [5]
  • per contare le GEC con un emocitometro[5]

Preparation Note

Preparato in cloruro di sodio 0,81% e fosfato di potassio bibasico 0,06%.

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Questo articolo
T614693595302643
Trypan Blue 0.4%, liquid, sterile-filtered, suitable for cell culture

Sigma-Aldrich

T8154

Trypan Blue

Trypan Blue powder, BioReagent, suitable for cell culture

Sigma-Aldrich

T6146

Trypan Blue

Trypan Blue 0.4%, suitable for microscopy

Sigma-Aldrich

93595

Trypan Blue

Trypan Blue ≥80% (HPLC), Dye content 60 %

Sigma-Aldrich

302643

Trypan Blue

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

technique(s)

cell culture | mammalian: suitable, tissue processing: suitable

technique(s)

microbe id | staining: suitable

technique(s)

microbe id | staining: suitable

concentration

0.4%

concentration

-

concentration

0.4%

concentration

-

application(s)

cell analysis

application(s)

cell analysis

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

diagnostic assay manufacturing
hematology
histology

form

liquid

form

powder

form

liquid

form

powder

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

shipped in

ambient

shipped in

-

shipped in

-

shipped in

-

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pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H350

Hazard Classifications

Carc. 1B

Classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number

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Questions

1–8 of 8 Questions  

  1. is this solution dissolved in PBS?

    1 answer

    1. As mentioned on the Product Detail Page at the DESCRIPTION section, under 'Preparation Note' , this product is prepared in 0.81% sodium chloride and 0.06% potassium phosphate, dibasic.

      Helpful?

  2. What is the stability of the product. How long we can use it as there is no expiry date mentioned in the COA

    1 answer

    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Helpful?

  3. Can Trypan blue, Product T8154, be used to discriminate between apoptotic and necrotic cells?

    1 answer

    1. Trypan blue will stain cells that have disrupted membranes.  It cannot differentiate between permeabilized membranes caused by apoptosis or necrosis.

      Helpful?

  4. Why is Trypan blue, Product T8154, used to determine cell viability?

    1 answer

    1. Trypan Blue is a vital stain recommended for use in estimating the proportion of viable cells in a population. The reactivity of this dye is based on the fact that the chromophore is negatively charged and does not react with the cell unless the membrane is damaged. Staining facilitates the visualization of cell morphology. Live (viable) cells do not take up the dye and dead (non-viable) cells do.

      Helpful?

  5. Trypan blue, Product T8154, contains a precipitate.  Is it still good to use?

    1 answer

    1. The trypan blue solution is still good to use.  It can be warmed to dissolve the precipitate or it can be filtered to remove the precipitate.

      Helpful?

  6. Why are there red fibers in Product T8154, Trypan blue solution?

    1 answer

    1. A report of fibers in Product T8154, Trypan blue solution is sufficiently common almost to be a characteristic of the product. These fibers are a cosmetic fault rather than one of functionality.  The presence of fibers may be more pronounced following cold storage or shipping.  This product should be stored at room temperature. Filtration to remove the fibers will not affect the use of the product.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. Do the cells need to be washed to remove the culture media prior to staining with Product T8154, Trypan Blue solution?

    1 answer

    1. Trypan Blue has a greater affinity for serum proteins than for cellular protein. If the background is too dark, cells should be pelleted and resuspended in protein-free medium or salt solution prior to counting.

      Helpful?

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