T7701
TargeTron™ Vector pJIR750ai
Expression Vector for Bacterial Gene Knockout
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
2 μg | Per conoscere la disponibilità, visualizza il carrello | CHF 183.00 |
Informazioni su questo articolo
NACRES:
NA.51
UNSPSC Code:
41106609
Servizio Tecnico
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Permettici di aiutartiQuality Segment
storage temp.
−20°C
General description
TargeTron™ vector pJIR750ai is a 10,262 bp expression vector intended for use with the TargeTron Gene Knockout System, Catalog Number TA0100. This circularized vector can be used for targeted gene knockouts in gram-positive bacteria such as Clostridium perfringens type A.1 Expression of the group II intron RNA is under the control of the β-2 toxin gene promoter, cpb2.1 As supplied, the pJIR750ai vector is re-targeted to the α-toxin (plc) gene. α-Toxin, a phospholipase C, is an extracellular toxin produced by all C. perfringens isolates.1 For validation in a specific C. perfringens strain, this vector can be used directly to knockout plc without any further modifications using the enclosed protocol. In order to re-target this vector to knockout other genes, the plc specific IBS-EBS fragment (350 bp) between the Hind III and BsrG I sites can be cut out and replaced with another gene specific fragment. For re-targeting protocols, refer to the TargeTron Gene Knockout System User Guide, Catalog Number TA0100, at sigma-aldrich.com.
Legal Information
TargeTron is a trademark of InGex, LLC
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Questo articolo | |||
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| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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