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T3809

Sigma-Aldrich

TRI reagent®

BD, For processing whole blood, plasma, or serum.

Sinonimo/i:

TRI Reagent® reagente per l’isolamento dell’RNA

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25 ML
CHF 85.70
100 ML
CHF 268.00
200 ML
CHF 475.00

CHF 85.70

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Tutte le immagini(1)

Scegli un formato

Cambia visualizzazione
25 ML
CHF 85.70
100 ML
CHF 268.00
200 ML
CHF 475.00

About This Item

Numero MDL:
MFCD00213058
Codice UNSPSC:
12352200
NACRES:
NA.52

CHF 85.70

Check Cart for Availability

Livello qualitativo

200

impiego

0.75 mL sufficient for 0.25 mL blood derivatives

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Descrizione generale

TRI Reagent BD is a quick and convenient reagent for use in the simultaneous isolation of RNA, DNA and protein from serum, plasma or whole blood. A convenient single-step liquid phase separation results in the simultaneous isolation of RNA, DNA and protein. This procedure is an adaptation of the single-step method reported by Chomczynski and Sacchi for total RNA isolation, and permits fast and efficient processing of blood derivatives.

TRI REAGENT BD performs well with large or small sample volumes, and many samples can be simultaneously extracted. TRI Reagent BD is a mixture of guanidine thiocyanate and phenol in a mono-phase solution. When a sample of blood derivatives is lysed with it, and chloroform or 1-bromo-3-chloropropane is added, the mixture separates into 3 phases: an aqueous phase containing the RNA, the interphase containing DNA and an organic phase containing proteins. Each component can then be isolated after separating the phases. 0.75 ml of TRI REAGENT BD processes 0.25 ml of blood derivatives.

This is one of the most effective methods for isolating total RNA and can be completed in only 1 hour starting with fresh cells. The procedure is very effective for isolating RNA molecules of all types from 0.1 to 15 kb in length. The resulting RNA is intact with little or no contaminating DNA or protein.
TRI Reagent® is a monophasic solution of phenol and guanidinium isothiocyanate.[1]

Applicazioni

Il Tri reagent consiste in una versione perfezionata del reagente per l'estrazione dell'RNA totale a singolo passaggio sviluppato da Chomczynski. Il metodo di isolamento dell'RNA basato su questo reagente è ampiamente utilizzato e di comprovata affidabilità per le successive applicazioni dell′RNA È ideale per l'isolamento rapido, economico ed efficiente dell'RNA totale o per l'isolamento simultaneo di RNA, DNA e proteine da campioni di origine umana, animale, vegetale, di lievito, batterici e virali.
TRI Reagent® has been used for the isolation of RNA.[2][3]
The resulting RNA can be used for Northern blots, mRNA isolation, in vitro translation, RNase protection assays, cloning and PCR.

Caratteristiche e vantaggi

  • Isolamento dell'RNA facilmente scalabile
  • Funziona con campioni di diversa origine: umana, vegetale, di lievito, batterica o virale
  • Ha una resa migliore rispetto ai tradizionali metodi guanidina tiocianato/cloruro di cesio

Note legali

TRI Reagent is a registered trademark of Molecular Research Center, Inc.

Avvertenze

Danger

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2

Organi bersaglio

Nervous system,Kidney,Liver,Skin

Rischi supp

EUH032

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

174.2 °F - closed cup

Punto d’infiammabilità (°C)

79 °C - closed cup

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Certificati d'analisi (COA)

Lot/Batch Number
MKCQ9520
MKCK7193
MKCH8720
MKCD4813
MKBX1805V

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Articoli

Genomic DNA Purification and Assessment

Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.

Questions

  1. When using Tri Reagent BD, do we need to add EDTA to blood samples to prevent coagulation?

    1 answer

    1. The Product Information available under More Documents or at this link (https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/436/845/t3809bul.pdf) provides the following instructions:

      I. Sample Preparation:

      1A. Serum: Add 0.25 ml of serum to 0.75 ml of TRI Reagent BD. Close the tube and shake the solution by hand or vortex, ensuring that mixing is thorough.

      1B. Whole Blood or Plasma: Add 0.2 ml of whole blood or plasma to 0.75 ml of TRI Reagent BD supplemented with 20 ml of 5 N acetic acid per 0.2 ml of whole blood or plasma. Close the tube and shake the solution by hand or vortex, ensuring that mixing is thorough.

      It is possible to use whole blood (blood that has been allowed to clot), serum, or plasma. Blood that has clotted would be referred to as a clot plus serum or simply as clotted blood. If you are freshly drawing the blood and adding immediately to the Tri Reagent, an anticoagulant would not be required. For serum samples, the blood is simply allowed to clot. The clot is spun down and serum is collected above the clot. If plasma is used, this indicates the blood is first drawn into either a tube containing an anticoagulant or drawn with a syringe and then transferred to a tube containing an anticoagulant. The blood is then centrifuged and the plasma is removed before adding to the Tri Reagent. We would not recommend allowing the blood to clot and then trying to add the clotted blood to the Tri Reagent.

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