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SRP5053

Sigma-Aldrich

NEK9 (1-979), (deleted fragment 346-731), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Sinonimo/i:

DKFZp434D0935, MGC138306, MGC16714, NEK8, NERCC, NERCC1

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Ricombinante

expressed in baculovirus infected Sf9 cells

Nome Commerciale

PRECISIO® Kinase

Saggio

≥70% (SDS-PAGE)

Forma fisica

buffered aqueous glycerol solution

Attività specifica

102-138 nmol/min·mg

PM

~115 kDa

N° accesso NCBI

NM_033116

Condizioni di spedizione

dry ice

Temperatura di conservazione

−70°C

Informazioni sul gene

human ... NEK9(91754)

Descrizione generale

NEK9 is a member of the NEK family and has high homology to NEK1, NEK3 and NEK4. NEK9 is activated during mitosis and binds specifically to RAN GTPase and is a substrate for CDC2 phosphorylation. NEK9 plays a role in the control of mitotic progression and is regulated by CDC2 and RAN GTPase. Overexpression of both active and inactive variants of NEK9 is toxic to cells and inhibits cell division causing abnormal nuclear morphologies. NEK9 can catalyze the phosphorylation of recombinant NEK6 and NEK7 in vitro leading to its activation. This suggests that NEK9 may be responsible for activation of NEK6 and NEK7 during mitosis.

Stato fisico

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Nota sulla preparazione

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Note legali

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Certificati d'analisi (COA)

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Joan Roig et al.
Genes & development, 16(13), 1640-1658 (2002-07-09)
The protein kinase NIMA is an indispensable pleiotropic regulator of mitotic progression in Aspergillus. Although several mammalian NIMA-like kinases (Neks) are known, none appears to have the broad importance for mitotic regulation attributed to NIMA. Nercc1 is a new NIMA-like
Christopher Belham et al.
The Journal of biological chemistry, 278(37), 34897-34909 (2003-07-04)
The Nek family of protein kinases in humans is composed of 11 members that share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying

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