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Documenti fondamentali

SRP3154

Sigma-Aldrich

sFRP-1 human

recombinant, expressed in HeLa cells, ≥97% (SDS-PAGE), ≥97% (HPLC), suitable for cell culture

Sinonimo/i:

SARP-2, Secreted Apoptosis Related Protein-2, Soluble Frizzled Related Protein-1

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20 μG
CHF 357.00

CHF 357.00

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20 μG
CHF 357.00

About This Item

Codice UNSPSC:
12352200
NACRES:
NA.77

CHF 357.00

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Origine biologica

human

Ricombinante

expressed in HeLa cells

Saggio

≥97% (HPLC)
≥97% (SDS-PAGE)

Stato

lyophilized

Potenza

0.3-0.5 μg/mL ED50

PM

31.0-36.0 kDa

Confezionamento

pkg of 20 μg

tecniche

cell culture | mammalian: suitable

Impurezze

<0.1 EU/μg endotoxin, tested

Colore

white

N° accesso UniProt

Q8N474

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... SFRP1(6422)

Descrizione generale

The gene SFRP1 (secreted frizzled related protein 1) is mapped to human chromosome 8p22–p12, a region frequently deleted in breast cancer. It is highly expressed in normal breast tissue.[1][2]

Azioni biochim/fisiol

Secreted Frizzled-related protein 1 (SFRP1) is a secreted inhibitor of Wnt signaling pathway. DNA damage or oxidative stress causes cellular senescence, which leads to overexpression of sfrp1. This protein induces senescence phenotypes via the inactivation of Wnt signaling and activation of the retinoblastoma (Rb) pathway.[3] Mutations or aberrant methylation in this gene impair the induction of senescence in several cancers, including breast cancer, bladder cancer, and colorectal tumorigenesis.[3][4][5][6]

Sequenza

SEYDYVSFQS DIGPYQSGRF YTKPPQCVDI PADLRLCHNV GYKKMVLPNL LEHETMAEVK QQASSWVPLL NKNCHAGTQV FLCSLFAPVC LDRPIYPCRW LCEAVRDSCE PVMQFFGFYW PEMLKCDKFP EGDVCIAMTP PNATEASKPQ GTTVCPPCDN ELKSEAIIEH LCASEFALRM KIKEVKKENG DKKIVPKKKK PLKLGPIKKK DLKKLVLYLK NGADCPCHQL DNLSHHFLIM GRKVKSQYLL TAIHKWDKKN KEFKNFMKKM KNHECPTFQS VFK

Stato fisico

Lyophilized from 10 mM Sodium Phosphate pH 7.0.

Ricostituzione

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

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Certificati d'analisi (COA)

Lot/Batch Number

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A M Goodwin et al.
Angiogenesis, 5(1-2), 1-9 (2003-01-29)
The Wnt signaling pathway regulates normal development as well as a variety of pathologies. Studies of the Wnt pathway have focused largely on very early development and on tumorigenesis. Recent observations point to a role for Wnt signaling in vessel
Methylation-associated silencing of SFRP1 with an 8p11-12 amplification inhibits canonical and non-canonical WNT pathways in breast cancers.
Yang ZQ
International Journal of Cancer. Journal International Du Cancer, 125, 1613-1621 (2009)
WNT pathway and mammary carcinogenesis: loss of expression of candidate tumor suppressor gene SFRP1 in most invasive carcinomas except of the medullary type.
Ugolini F
Oncogene, 20, 5810-5817 (2001)
Xiaobin Wang et al.
Oncology letters, 4(2), 334-338 (2012-07-31)
The aims of this study were to determine the methylation and expression status of secreted Frizzled-related protein 1 (SFRP1) in bladder cancer, to explore the mechanisms involved and to study the role of SFRP1 in the pathogenesis of bladder cancer.
Germaine M Caldwell et al.
Cancer research, 64(3), 883-888 (2004-02-12)
Regions of the short arm of chromosome 8 are deleted frequently in a range of solid tumors, indicating that tumor suppressor genes reside at these loci. In this study, we have examined the properties of the Wnt signaling antagonist secreted
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