SRP2034
Topo I (N1-197) (NTD) human
recombinant, expressed in insect cells, ≥85% (SDS-PAGE)
Sinonimo/i:
TOPI
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.26
Origine biologica
human
Ricombinante
expressed in insect cells
Saggio
≥85% (SDS-PAGE)
Forma fisica
frozen liquid
PM
~25.3 kDa
Confezionamento
pkg of 5 μg
Condizioni di stoccaggio
avoid repeated freeze/thaw cycles
Concentrazione
1000 μg/mL
Colore
clear colorless
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... TOP1(7150)
Azioni biochim/fisiol
Human DNA topoisomerase I is the best studied of the DNA topoisomerase family. It catalyzes the relaxation of both positive and negative supercoils without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. The N-terminal 214 amino acids of topoisomerase I comprise a highly charged N-terminal domain (NTD) involved in protein-protein interactions with a number of cellular proteins, including SV40 T antigen, nucleolin, SR proteins, p53 and other transcription factors.
Stato fisico
Clear and colorless frozen liquid solution
Nota sulla preparazione
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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D Gai et al.
Journal of virology, 74(11), 5224-5232 (2000-05-09)
Topoisomerase I (topo I) is required for releasing torsional stress during simian virus 40 (SV40) DNA replication. Recently, it has been demonstrated that topo I participates in initiation of replication as well as in elongation. Although T antigen and topo
Yinghui Mao et al.
Proceedings of the National Academy of Sciences of the United States of America, 99(3), 1235-1240 (2002-01-24)
The nonconserved, hydrophilic N-terminal domain of eukaryotic DNA topoisomerase I (topo I) is dispensable for catalytic activity in vitro but essential in vivo. There are at least five putative nuclear localization signals and a nucleolin-binding signal within the first 215
P Haluska et al.
Nucleic acids research, 26(7), 1841-1847 (1998-05-30)
We have attempted to identify human topoisomerase I-binding proteins in order to gain information regarding the cellular roles of this protein and the cytotoxic mechanisms of the anticancer drug camptothecin, which specifically targets topoisomerase I. In the course of this
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