SRP0238

cMet Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

• Sinonimo/i: HGFR, Hepatocyte growth factor receptor, RCCP2, Scatter factor receptor, met Proto-oncogene
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥60% (SDS-PAGE)
• Forma fisica: aqueous solution
• PM: 74 kDa
• Confezionamento: pkg of 10 μg
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: X54559
• N° accesso UniProt: P08581
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... MET(4233)

Descrizione

Descrizione generale

Human cMet (GenBank Accession No. X54559), (amino acids 956-1390) with N-terminal GST tag, MW = 74 kDa, expressed in an Baculovirus infected Sf9 cell expression system.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors and selectivity profiling.

Definizione di unità

One unit is defined as the amount of enzyme that will transfer 1 pmol phosphate to Tyr substrate per minute at pH 7.4 and 30°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 130 mM NaCl, 0.05% Tween-20, 3 mM DTT and 20% glycerol.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.