SRP0130

EZH1/EED/SUZ12  human

recombinant, expressed in baculovirus infected insect cells

• Sinonimo/i: ENX-2, EZH1, Enhancer of zeste homolog 1, KMT6B, Lysine N-methyltransferase 6B
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Origine biologica: human
• Ricombinante: expressed in baculovirus infected insect cells
• Saggio: ≥80% (SDS-PAGE)
• Stato: aqueous solution
• PM: 51 kDa (EED); 86 kDa (EZH1); 87 kDa (SUZ12)
• Confezionamento: pkg of 50 μg
• Concentrazione: >0.02 mg/mL
• N° accesso NCBI: NM_001991 (EZH1); NM_003797 (EED); NM_015355 (SUZ12)
• N° accesso UniProt: Q92800
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −70°C
• Informazioni sul gene: human ... EZH1(2145)

Descrizione

Descrizione generale

Complex of: Human EZH1, GenBank Accession No. NM_001991, amino acids 2-end, with N-terminal His tag, MW = 86 kDa. Human EED, NM_003797, amino acids with N-terminal DDDDK tag (FLAG), MW = 51 kDa. Human SUZ12,. NM_015355, amino acids 2-end, with N-terminal His tag.

Applicazioni

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Definizione di unità

One unit is defined as the amount of enzyme required to methylate 1 pmol of substrate/min at 37°C.

Stato fisico

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide and 10% glycerol.

Nota sulla preparazione

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.