SHPH01

MISSION^® LentiPlex^® Human Pooled shRNA Library

For Rapid, Convenient Genome-wide shRNA Screens

• Sinonimo/i: Human shRNA Library, LentiPlex shRNA Library
• NACRES: NA.51
• UNSPSC Code: 41105904
• Concentration: ≥5x10⁸ VP/ml (via p24 assay)
• Technique(s): capture ELISA: 5 × 10⁸ TU/mL using p24

Proprietà

• Quality Segment: 200
• product line: MISSION^®
• concentration: ≥5x10⁸ VP/ml (via p24 assay)
• technique(s): capture ELISA: 5 × 10⁸ TU/mL using p24
• shipped in: dry ice
• storage temp.: −70°C

Descrizione

General description

MISSION LentiPlex Pooled Libraries bring the power of the MISSION TRC shRNA collection together with Sigma¢s lentiviral manufacturing expertise to enable genome-wide RNAi screens at your bench-top. The LentiPlex pooled system provides enhanced delivery and long-term gene silencing in non-dividing and primary cell lines allowing unlimited discovery potential. The convenient format now makes rapid whole-genome RNAi screens accessible to any researcher with minimal reagent, time or capital equipment investment.

The MISSION LentiPlex Human shRNA Pooled Library is a genome-wide lentiviral pool produced using a proprietary process. The library consists of over 75,000 shRNA constructs from the TRC collection targeting 15,000+ human genes. Each library is tested for shRNA representation before product release to ensure robust library coverage. The library is provided in ready-to-use lentiviral format at titers of at least 5 x 108 TU/ml via p24 assay and is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Amplification and sequencing primers are also provided for downstream target identification.

The library is pre-divided into ten subpools of approximately 8,000 shRNA constructs each. Each library is enough material for six screens at 100X representation.

Please visit sigma.com/lentiplex to learn more and see example data and uses.

Application

MISSION^® LentiPlex^® Human Pooled shRNA Library has been used to transfect human colon adenocarcinoma cell line HT29 for shRNA screening and bioinformatics analysis.

Other Notes

Deconvolution of shRNA Pools
Sigma′s deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.

• Next-generation sequencing of clonesgives a precise number of individual clone occurrence within a pooled shRNA sample
• Comprehensive, reproducible results from pooled shRNA screens
• Statistically robust and information-rich data

Contact your local Sigma sales representative for more information or submit an inquiry to MISSION RNAi

Legal Information

Lentiviral License Agreement

LentiPlex is a registered trademark of Merck KGaA, Darmstadt, Germany

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Classe di stoccaggio: 12 - Non Combustible Liquids
• Che succede?: WGK 3
• Punto di infiammabilità (°F): Not applicable
• Punto di infiammabilità (°C): Not applicable