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SHC312

Sigma-Aldrich

MISSION® 1X LacO Inducible Non-Target shRNA Control Plasmid DNA

Does not target any known genes

Sinonimo/i:

MISSION® Control Vectors

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About This Item

Numero di Ballschmiter:
12000000
Numero MDL:
MFCD07785395
Codice UNSPSC:
41106609
NACRES:
NA.51

Nome Commerciale

MISSION®

Concentrazione

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Categorie correlate

Descrizione generale

Sigma® is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC.

The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-Β-D-thio-galactoside), an analogue of lactose, LacI binds to LacO preventing expression of the shRNA. When IPTG is present, the allosteric LacI repressor changes conformation, releasing itself from lacO modified human U6 promoter, and subsequently allows expression of the shRNA.

The vector contains an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The 1X LacO Inducible Non-Target shRNA Control is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Applicazioni

To see more application data, protocols, vector maps visit sigma.com/shrna.

Note legali

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Sigma is a registered trademark of Sigma-Aldrich Co. LLC

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

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Articoli

Efficient Gene Silencing with MISSION® TRC shRNA

RNAi Consortium (TRC): Collaborative effort among academic labs and biotech/pharma institutes advancing RNA interference research.

Contenuto correlato

Inducible shRNA Vectors

Explore the benefits of IPTG-inducible vectors through performance data. These vectors offer regulated expression, which is important when studying essential and lethal genes.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.