SAB4702131

Monoclonal Anti-CD22 FITC produced in mouse

• Sinonimo/i: SIGLEC-2, SIGLEC2
• Codice UNSPSC: 12352203

Proprietà

• Coniugato: FITC conjugate
• Livello qualitativo: 100
• Tipo di anticorpo: primary antibodies
• Clone: S-HCL-1, monoclonal
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: human
• tecniche: flow cytometry: suitable
• Isotipo: IgG2bκ
• N° accesso UniProt: P20273
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CD22(933)

Descrizione

Descrizione generale

CD22, also known as Siglec-2 (sialic acid-binding immunoglobulin-like lectin-2) is a transmembrane glycoprotein binding alpha2,6-linked sialic acid-bearing ligands. Intracellular domain of CD22 recruits protein tyrosine phosphatase SHP-1 through the immunoreceptor tyrosine-based inhibitory motifs (ITIMs), thus setting a treshold for B cell receptor-mediated activation. CD22 also regulates B-cell response by involvement in controlling the CD19/CD21-Src-family protein tyrosine kinase amplification pathway and CD40 signaling. CD22 exhibits hallmarks of clathrin-mediated endocytic pathway.

Specificità

The mouse monoclonal antibody S-HCL-1 (also known as S-HCL1) recognizes an extracellular epitope of CD22 (BL-CAM), a 130 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes; it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes.

Immunogeno

Whole hairy cell leukemia cells and membrane preparation

Stato fisico

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Nota sulla preparazione

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Stoccaggio e stabilità

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Altre note

The antibody solution should be gently mixed before use.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable