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SAB4200544

Sigma-Aldrich

Anti-Synaptophysin antibody, Mouse monoclonal

clone SVP-38, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-MRXSYP, Monoclonal Anti-SYP, Monoclonal Anti-Synaptophysin1, Monoclonal Anti-Synaptoporin, Monoclonal Anti-Syp1, Monoclonal Anti-p38

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Livello qualitativo

Coniugato

unconjugated

Forma dell’anticorpo

purified immunoglobulin

Tipo di anticorpo

primary antibodies

Clone

SVP-38, monoclonal

Stato

buffered aqueous solution

PM

antigen 38 kDa

Reattività contro le specie

rat, human

Concentrazione

~1.0 mg/mL

tecniche

immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded rat cerebellum.
indirect immunofluorescence: 10-20 μg/mL using B35 cells.
western blot: 1-2 μg/mL using newborn rat brain extracts.

Isotipo

IgG1

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Informazioni sul gene

human ... SYP(6855)

Descrizione generale

Anti-Synaptophysin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma SVP-38 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synaptosome preparation from rat retina. Synaptophysin is a glycoprotein containing four transmembrane domains with both N and C termini on the cytoplasm face.
Synaptophysin (SYP) is encoded by the gene mapped to human chromosome Xp11.23-p11.22. The encoded protein has a molecular mass of 38kDa and is exclusively localized to the membrane of presynaptic vesicles.

Specificità

Anti-Synaptophysin antibody, Mouse monoclonal recognizes rat, human, guinea-pig, and pig synaptophysin

Immunogeno

synaptosome preparation from rat retina. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.

Applicazioni

Anti-Synaptophysin antibody, Mouse monoclonal has been used in following immunochemical techniques:
  • Immunoblotting
  • Immunohistochemistry
  • Immunofluorescence

Azioni biochim/fisiol

Synaptophysin (SYP) facilitates neurotransmitter release and synaptic plasticity. It is also implicated in synaptic vesicle formation and exocytosis. Mutation in the gene increases the risk of susceptibility to schizophrenia. It is widely used as a potential marker to study the circulation of synapses in the brain and to discover the basic features of the life cycle of synaptic vesicles (SVs).
Synaptophysin has also been implicated in the recycling of synaptic vesicles by associating with dynamin I, a GTPase required for endocytosis.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Raccomandato

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Mikihito Shibata et al.
Nature, 598(7881), 489-494 (2021-10-03)
The similarities and differences between nervous systems of various species result from developmental constraints and specific adaptations1-4. Comparative analyses of the prefrontal cortex (PFC), a cerebral cortex region involved in higher-order cognition and complex social behaviours, have identified true and
Identification and localization of synaptophysin, an integral membrane glycoprotein of Mr 38,000 characteristic of presynaptic vesicles
Wiedenmann B and Franke WW
Cell, 41, 1017-1028 (1985)
Neuronal connections of direct and indirect pathways for stable value memory in caudal basal ganglia
Amita H, et al.
The European Journal of Neuroscience, 49(5), 712-725 (2019)
Synaptophysin: leading actor or walk-on role in synaptic vesicle exocytosis?
Valtorta F
Bioessays, 26, 445-453 (2004)
Association between SYP with attention-deficit/hyperactivity disorder in Chinese Han subjects: differences among subtypes and genders.
Liu L
Psychiatry Research, 210, 308-314 (2013)

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