SAB4200352

Anti-DLC1 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody

• Sinonimo/i: Anti-ARHGAP7, Anti-Deleted in Liver Cancer 1, Anti-FLJ21120, Anti-HP, Anti-STARD12, Anti-p122-RhoGAP
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Stato: buffered aqueous solution
• PM: antigen ~200 kDa
• Reattività contro le specie: human
• Convalida avanzata: recombinant expression; Learn more about Antibody Enhanced Validation
• Concentrazione: ~1.5 mg/mL
• tecniche: western blot: 1-2 μg/mL using lysates of HEK-293T cells overexpressing human DLC1.
• N° accesso UniProt: Q96QB1
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... DLC1(10395)

Descrizione

Descrizione generale

Deleted in liver cancer 1 (DLC1) is a candidate tumor suppressor gene, originally obtained from human hepatocellular carcinoma (HCC). It is mapped to the human chromosome location 8p21-22. The DLC1 gene is majorly expressed in normal human tissues. It contains three major functional domains, sterile α-motif (SAM), Rho guanosine triphosphatase-activating protein (RhoGAP), and steroidogenic acute regulatory-related lipid transfer (START) domains. The N-terminal region of DLC1 localizes to focal adhesions by binding to Src Homology 2 (SH2) domains on tensins.

Specificità

Anti-DLC1(C-terminal) specifically recognizes human DLC1.

Immunogeno

synthetic peptide corresponding to a sequence at the C-terminal of human DLC1, conjugated to KLH. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Applicazioni

Anti-DLC1 (C-terminal) antibody produced in rabbit may be used in immunoblotting.

Azioni biochim/fisiol

Deleted in liver cancer 1 (DLC1) interacts with tensin2, a focal adhesion protein, localized at the end of stress fibers, which plays key roles in cytoskeletal organization and cell signaling. Mutations in the focal adhesion targeting (FAT) region of DLC1 reduces its expression and function. The focal adhesion localization of DLC1 is critical for cell motility and morphology. It is also critical for the tumor suppressor activity of DLC1 and under expressed in human hepatocellular carcinoma (HCC) and a variety of cancer types.

Stato fisico

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable