S5446
Anti-SUMO-1 (C-terminal) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
Sinonimo/i:
Anti-SMT3H3
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About This Item
Prodotti consigliati
Origine biologica
rabbit
Coniugato
unconjugated
Forma dell’anticorpo
affinity isolated antibody
Tipo di anticorpo
primary antibodies
Clone
polyclonal
Forma fisica
buffered aqueous solution
PM
antigen 14 kDa
Reattività contro le specie
human
tecniche
microarray: suitable
western blot: 1-2 μg/mL using nuclear extract of the human epitheloid carcinoma HeLa cell line
N° accesso UniProt
Condizioni di spedizione
dry ice
Temperatura di conservazione
−20°C
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
cow ... SUMO1(614967)
human ... SUMO1(7341)
mouse ... Sumo1(22218)
rat ... Sumo1(301442)
Descrizione generale
Small ubiquitin-related modifier 1 (SUMO-1) is a highly conserved small ubiquitin-related modifier, also known as sentrin. SUMO-1 gene is mapped to human chromosome 2q33.1.
Specificità
Anti-SUMO-1 (C-terminal) recognizes unconjugated SUMO-1 (14 kDa), SUMO-1-fusion protein, as well as proteins covalently conjugated to SUMO-1, eg. RanGAP1, 90kDa
Immunogeno
synthetic peptide corresponding to amino acids 86-97 located at the C-terminus of human SUMO-1, conjugated to KLH. This sequence is identical in many species including rat, mouse, bovine, chicken, and Xenopus, and highly conserved (single amino acid substitution) in C. elegans SMT3. The sequence has only 66% homology to human SUMO-2 and SUMO-3.
Applicazioni
Anti-SUMO-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting.
Azioni biochim/fisiol
Small ubiquitin-related modifier 1 (SUMO-1) is covalently conjugated to the lysine side in proteins through its C-terminal glycine residue by an isopeptide bond. Their conjugation with cellular proteins has been correlated wide variety of biological processes including inflammation, oncogenesis nuclear transport, cell cycle control and viral infection response. SUMO-1 serves as a substrate for Ran GTPase-activating protein (RanGAP1). Unmodified RanGAP1 is present in the cytoplasm, suggesting that modification by SUMO-1 may target RanGAP1 to the nuclear pore complex (NPC). Haploinsufficiency in the SUMO-1 gene is implicated in the Cleft Lip and Palate.
Stato fisico
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
Stoccaggio e stabilità
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Aleksandra Fergin et al.
PLoS genetics, 18(6), e1009978-e1009978 (2022-06-07)
The sumoylation (SUMO) pathway is involved in a variety of processes during C. elegans development, such as gonadal and vulval fate specification, cell cycle progression and maintenance of chromosome structure. The ubiquitous expression and pleiotropic effects have made it difficult
Soraya Beiraghi et al.
American journal of human genetics, 81(1), 180-188 (2007-06-15)
Nonsyndromic cleft lip with or without cleft palate (NSCL/P) is one of the most common congenital facial defects, with an incidence of 1 in 700-1,000 live births among individuals of European descent. Several linkage and association studies of NSCL/P have
H Saitoh et al.
The Journal of biological chemistry, 275(9), 6252-6258 (2000-02-29)
Post-translational modification marked by the covalent attachment of the ubiquitin-like protein SUMO-1/SMT3C has been implicated in a wide variety of cellular processes. Recently, two cDNAs encoding proteins related to SUMO-1 have been identified in human and mouse. The functions and
Fowzan S Alkuraya et al.
Science (New York, N.Y.), 313(5794), 1751-1751 (2006-09-23)
The posttranslational modification sumoylation can have multiple effects on its substrate proteins. We studied a patient with isolated cleft lip and palate and a balanced chromosomal translocation that disrupts the SUMO1 (small ubiquitin-related modifier) gene, resulting in haploinsufficiency. In mouse
Xiuling Li et al.
Development (Cambridge, England), 139(23), 4321-4329 (2012-11-08)
In vertebrates, establishment of the hematopoietic stem/progenitor cell (HSPC) pool involves mobilization of these cells in successive developmental hematopoietic niches. In zebrafish, HSPCs originate from the ventral wall of the dorsal aorta (VDA), the equivalent of the mammalian aorta-gonad-mesonephros (AGM).
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