RECOMT
Thrombin CleanCleave™ Kit
Sinonimo/i:
Thrombin Cleavage Kit
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.56
Prodotti consigliati
Forma fisica
suspension
PM
37 kDa
tecniche
protein purification: suitable
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
Descrizione generale
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Applicazioni
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Caratteristiche e vantaggi
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Quantità
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Note legali
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
Solo come componenti del kit
N° Catalogo
Descrizione
- Thrombin Cleavage Buffer, 10× 10 mL
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Articoli
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Protocolli
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
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