RECOMT
Thrombin CleanCleave™ Kit
Sinonimo/i:
Thrombin Cleavage Kit
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 kit | Per conoscere la disponibilità, visualizza il carrello | CHF 332.00 |
Informazioni su questo articolo
NACRES:
NA.56
UNSPSC Code:
12352200
Servizio Tecnico
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Permettici di aiutartiform
suspension
Quality Segment
mol wt
37 kDa
technique(s)
protein purification: suitable
shipped in
wet ice
storage temp.
2-8°C
General description
The Thrombin CleanCleave Kit contains a 50% suspension fo thrombin-agarose produced by immobilizing bovine thrombin and is designed for cleavage of recombinant fusion proteins
Application
For cleaving a tag from a recombinant fusion protein which contains the thrombin recognition sequence. Thrombin-agarose is compatible with recombinant proteins expressed in various types of expression systems.
Features and Benefits
- Fast, efficient cleavage in as little as 2 hours
- Thrombin is covalently bound to agarose for easy removal.
- The robust cleavage reaction is effective at temperatures from 4 °C to 37 °C and over a wide range of pH and ionic strengths.
- Cleave tags even in the presence of 0.1% Triton™, 1 M urea or 5 mM EDTA
- Thrombin-agarose is reusable.
Preparation Note
200 μl of a 50% slurry of thrombin-agarose cleaves >85% of 1 mg of fusion protein.
Legal Information
CleanCleave is a trademark of Sigma-Aldrich Co. LLC
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Questo articolo | |||
|---|---|---|---|
| description - | description human recombinant,expressed in HEK 293 cells, ≥5000 units/mL | description BioUltra, recombinant, expressed in HEK 293 cells, aqueous solution, ≥95% (SDS-PAGE) | description - |
| technique(s) protein purification: suitable | technique(s) protein extraction: suitable | technique(s) - | technique(s) protein extraction: suitable |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 400 |
| mol wt 37 kDa | mol wt - | mol wt 37.4 kDa | mol wt - |
| shipped in wet ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
| form suspension | form - | form aqueous solution | form liquid |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −70°C | storage temp. −20°C |
Solo come componenti del kit
N° Catalogo
Descrizione
- Thrombin Cleavage Buffer, 10× 10 mL
Classe di stoccaggio
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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