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Merck

RAB0431

Mouse Shh-N ELISA Kit

for serum, plasma and cell culture supernatant

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Informazioni su questo articolo

NACRES:
NA.32
UNSPSC Code:
41116158
Gene information:
mouse ... Shh(20423)
Input:
sample type cell culture supernatant(s)
sample type serum
sample type plasma
Species reactivity:
mouse
Storage temp.:
−20°C
Shipped in:
wet ice
Servizio Tecnico
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species reactivity

mouse

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 5 pg/mL
standard curve range: 4.1-1000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Shh(20423)

General description

The Mouse Shh-N (Sonic Hedgehog N Terminus) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Shh-N in serum, plasma, cell culture supernatants and urine.

Immunogen

Recombinant Mouse Sonic Hedgehog (N-terminus)

Application

For research use only. Not for use in diagnostic procedures.
Mouse Shh-N ELISA Kit has been used to measure serum sonic hedgehog (Shh) level.

Biochem/physiol Actions

Sonic hedgehog (Shh) is a secreted glycoprotein involved in embryonic morphogenic development of several tissues. It has a role in fracture healing and regeneration. In the canonical pathway, the protein associates with its receptor Patched (Ptc-1), de-represses Smoothened (Smo) and activates the Gli family of transcription factors. Mutations in the Shh pathway have been linked to developmental diseases and cancer. Shh plays a vital role in axon growth and guidance throughout the brain development. Shh stimulates neurite outgrowth by overcoming neurite growth inhibitory environment associated with glial scar.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.


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I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS & Determinazione del prezzo

  • ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
    SDS

  • ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
    SDS

  • ELISA Stop Solution (Item I)
    SDS

  • ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
    SDS

  • 20X Wash Buffer (Item B)
    SDS

Pittogrammi

Health hazard

Parola di avvertenza

Danger

Codici di pericolo

Avvertenze di sicurezza

Hazard Classifications

Resp. Sens. 1

Classe di stoccaggio

10 - Combustible liquids



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Certificati d'analisi (COA)

Lot/Batch Number

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