RAB0327

Human Insulin ELISA Kit

for serum, plasma, cell culture supernatants and urine

• Sinonimo/i: Insulin
• Codice UNSPSC: 41116158
• NACRES: NA.32

Proprietà

• Reattività contro le specie: human
• Confezionamento: kit of 96 wells (12 strips x 8 wells)
• tecniche: ELISA: suitable; capture ELISA: suitable
• input: sample type plasma; sample type serum; sample type cell culture supernatant(s); sample type urine
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 4 μIU/mL; standard curve range: 4.69-300 μIU/mL
• Metodo di rivelazione: colorimetric
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... INS(3630)

Descrizione

Descrizione generale

The Human Insulin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Insulin and Proinsulin in serum, plasma, cell culture supernatants and urine.

Immunogeno

Recombinant Human Insulin

Applicazioni

For research use only. Not for use in diagnostic procedures.

Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum and HAMECs (human adipose microvascular endothelial cells) which were isolated from human visceral fat of nondiabetic patients.[1][2]
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Human Insulin ELISA Kit has been used:

• to measure the blood levels of human insulin and proinsulin in the transgenic mice[3]
• to measure serum insulin indaibetic rats [4]
• to measure insulin in Multiple Sclerosis patients[5]

Azioni biochim/fisiol

Insulin is responsible for two types of actions, excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis.[6] Insulin plays a vital role in regulation of blood glucose level within the normal range. Abnormal insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycemia.[7] Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin.[8] Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding.[9] Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.[10]

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

• RABELADA ELISA 1X Assay/Sample Diluent Buffer A (Item D1)
• RABELADB ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
• RABSTOP3 ELISA Stop Solution (Item I)
• RABTMB3 ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
• RABWASH4 20X Wash Buffer (Item B)

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Warning
• Indicazioni di pericolo: H290
• Consigli di prudenza: P234 - P390
• Classi di pericolo: Met. Corr. 1
• Codice della classe di stoccaggio: 8A - Combustible corrosive hazardous materials