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RAB0145

Sigma-Aldrich

Human Dkk-3 ELISA Kit

for serum, plasma, cell culture supernatants and urine

Sinonimo/i:

Dickkopf-related protein 3, Dkk-3

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About This Item

Codice UNSPSC:
41116158
NACRES:
NA.32

Reattività contro le specie

human

Confezionamento

kit of 96 wells (12 strips x 8 wells)

tecniche

ELISA: suitable
capture ELISA: suitable

input

sample type cell culture supernatant(s)
sample type plasma
sample type urine
sample type serum

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 3 pg/mL
standard curve range: 2.74-2000 pg/mL

Metodo di rivelazione

colorimetric

Condizioni di spedizione

wet ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... DKK3(27122)

Descrizione generale

The Human Dickkopf homolog 3 (DKK3) enzyme-linked immunosorbent assay (ELISA) kit is an in vitro enzyme-linked immunosorbent assay used for the quantitative measurement of human DKK-3 in serum, plasma, cell culture supernatants, and urine. Dickkopf homolog 3 (DKK3) protein belongs to the Dkk family. The DKK3 gene acts as a tumor suppressor gene and is suppressed in several human cancers. This gene is mapped on the human chromosome at 11p15.3.

Immunogeno

Recombinant Human DKK3

Applicazioni

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Human Dkk-3 ELISA Kit has been used to evaluate Dickkopf homolog 3 (DKK3) from conditioned media. It has also been used to determine DKK3 levels in urine samples.

Altre note

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS

  • RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS

  • RABSTOP3ELISA Stop Solution (Item I)SDS

  • RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS

  • RABWASH420X Wash Buffer (Item B)SDS

Pittogrammi

Corrosion

Avvertenze

Warning

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Met. Corr. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Francesca Valdora et al.
International journal of molecular sciences, 14(4), 7492-7505 (2013-04-10)
Medulloblastoma (MB) is a malignant pediatric brain tumor arising in the cerebellum consisting of four distinct subgroups: WNT, SHH, Group 3 and Group 4, which exhibit different molecular phenotypes. We studied the expression of Dickkopf (DKK) 1-4 family genes, inhibitors
Kazuyasu Kobayashi et al.
Gene, 282(1-2), 151-158 (2002-01-30)
The human REIC gene is a recently found mortalization-related gene and a candidate tumor suppressor gene expression of which is largely attenuated in many immortalized and tumor-derived cell lines (Biochem. Biophys. Res. Commun. 268 (2000) 20-24). To gain insight into
Kenta Torigoe et al.
Medicina (Kaunas, Lithuania), 57(6) (2021-07-03)
Background and Objectives: Urinary levels of dickkopf-3 (DKK-3) are associated with poor renal survival in patients with non-dialytic chronic kidney disease. However, it remains unknown whether urinary DKK-3 levels can predict residual renal function (RRF) decline in patients undergoing peritoneal
Jun Yu et al.
Cancer, 115(1), 49-60 (2008-12-04)
Abnormal activation of the Wnt/beta-catenin signaling pathway is common and critical in the pathogenesis of digestive cancers. In this study, the authors investigated the promoter methylation of the dickkopf homolog 3 gene Dkk-3 in these cancers and its prognostic significance
Shuang Zhao et al.
Frontiers in oncology, 10, 600322-600322 (2021-01-12)
Dkk3 has been discovered during comparison of immortalized and parental cells. Its expression has been shown to reduce colony formation and induce apoptosis of cancer cells, acting as a tumor suppressor. Herein, we demonstrate that Dkk3 overexpression or protein treatment

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