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R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Sinonimo/i:

Transfer RNA, tRNA

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About This Item

Numero CAS:
9014-25-9
Numero MDL:
MFCD00132200
Codice UNSPSC:
12352106
NACRES:
NA.55

Grado

for molecular biology

Livello qualitativo

300

Tipo

Type V

Forma fisica

lyophilized powder

Attività specifica

15-19 units/mg solid

Condizioni di stoccaggio

desiccated

Solubilità

H2O: ~2 mg/mL, clear to hazy

Temperatura di conservazione

2-8°C

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Descrizione generale

Wheat germ tRNA contains many different specific tRNA molecules.

Applicazioni

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Componenti

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Definizione di unità

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Sigma-Aldrich

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