No, all the cocktails are EDTA-free. Alternative metalloprotease inhibitors are included in the cocktail when needed, depending on the sample type. EDTA can be added if it does not interfere with the downstream purification or activity of your protein of interest. Please explore the reagent offerings below for recommended, ready-to-use EDTA products.
PIC0002
Protease Inhibitor Cocktail
ReadyShield® ready-to-use solution, for the inhibition of serine, cysteine, acid proteases and aminopeptidases, for use with mammalian cell and tissue extracts, Non-freezing solution
Sinonimo/i:
Protease inhibitor cocktail, general use protease inhibitor cocktail
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 114.00 |
5 mL | Per conoscere la disponibilità, visualizza il carrello | CHF 461.00 |
Informazioni su questo articolo
UNSPSC Code:
12352200
NACRES:
NA.54
Form:
ready-to-use solution
Solubility:
water: soluble
Storage temp.:
−20°C
CHF 114.00
Per conoscere la disponibilità, visualizza il carrello
Servizio Tecnico
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ReadyShield® Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, Non-freezing solution
description
Mammalian Cell and Tissue Extracts
Quality Segment
form
ready-to-use solution
quality
200
packaging
pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial)
input
mammalian sample(s)
solubility
water: soluble
storage temp.
−20°C
General description
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts. It contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases. The cocktail has been tested on extracts from various animal tissues such as human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells.
This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield PIC0002 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8340.
This cocktail is supplied as a ready-to-use solution using a proprietary non-freezing formulation.
ReadyShield PIC0002 is a non-freezing formulation that contains the same inhibitors as is the DMSO based cocktail P8340.
Features and Benefits
- Does not freeze in storage for quick, convenient use.
- Tailored for Mammalian cell and tissue extracts.
- Suggested as 100 X solution.
- Reclosable screw-top vials.
- Can be stored in cooler for approx. 1 month or room temperature for a week.
Preparation Note
The recommended dilution of the cocktail in the biological extract is 1 ml of the cocktail in 100 ml of lysate derived for example from 20g (wet weight) of bovine liver or 109 CHO cells
Other Notes
For life science research only. Not for use in diagnostic procedures.
Legal Information
ReadyShield is a registered trademark of Merck KGaA, Darmstadt, Germany
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Questo articolo | |||
|---|---|---|---|
| description for use with mammalian cell and tissue extracts, Non-freezing solution | description non-freezing solution | description Safe for Proteomic Mass Spectrometry applications | description - |
| form ready-to-use solution | form ready-to-use solution | form ready-to-use solution | form DMSO solution |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| solubility water: soluble | solubility water: soluble | solubility water: soluble | solubility water: soluble |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging pkg of 1 mL (in amber polypropylene vial), pkg of 5 mL (in amber polypropylene vial) | packaging - | packaging pkg of 1 ml, 5 ml × |
| quality 200 | quality - | quality - | quality - |
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signalword
Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Classe di stoccaggio
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Do ReadyShield® inhibitor cocktails include EDTA?
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What is the recommended storage temperature for ReadyShield® products?
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The product is stable at -20 °C for 2 years from the assay date, which can be found on the lot specific CoA. The solution will not freeze in -20 °C storage and can be used directly from the freezer. Our experts have collected additional stability information that show ReadyShield® products are stable for at least one month at 4 °C, or for at least one week at 25 °C (room temperature).
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