MTOX1105
MRP3 Knockout HepaRG™ Cells
human female liver (hepatocarcinoma and hepatitis C)
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| Voi/SKU | Disponibilità | Prezzo |
|---|---|---|
1 vial | Per conoscere la disponibilità, visualizza il carrello | CHF 1050.00 |
Informazioni su questo articolo
UNSPSC Code:
12352207
NACRES:
NA.81
Biological source:
human female liver (hepatocarcinoma and hepatitis C)
Servizio Tecnico
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MRP3 Knockout HepaRG™ Cells, 1 vial
biological source
human female liver (hepatocarcinoma and hepatitis C)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... MRP3(8714)
General description
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MRP3 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
Application
See technical bulletin for detailed protocols
Features and Benefits
HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These the cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.
- Sigma′s HepaRG MRP3 Knockout (KO) allows investigations of drug-transporter interactions involving MRP3 in the liver.
Zinc finger nucleases (ZFN) mediated Knock-out of ABCC3 (MRP3) gene.
- The frame-shift mutation of ABCC3 gene was confirmed by fragment length analysis and DNA sequencing.
- Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.
Legal Information
These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company
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Questo articolo | |||
|---|---|---|---|
| description 1 vial | description 1 vial | description 1 vial | description 1 vial |
| biological source human female liver (hepatocarcinoma and hepatitis C) | biological source human female liver (hepatocarcinoma and hepatitis C tumor) | biological source human female liver (Source Disease: Hepatocarcinoma and Hepatitis C) | biological source human female liver (Source Disease: Hepatocarcinoma and Hepatitis C) |
| storage temp. −196°C | storage temp. −196°C | storage temp. −196°C | storage temp. −196°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| OMIM accession no. | OMIM accession no. | OMIM accession no. - | OMIM accession no. |
| Gene Information human ... MRP3(8714) | Gene Information human ... OATP1B3(28234) | Gene Information human ... ABCB1(5243) | Gene Information human ... NR1I2(1337040) |
Classe di stoccaggio
12 - Non Combustible Liquids
Che succede?
WGK 3
Punto di infiammabilità (°F)
Not applicable
Punto di infiammabilità (°C)
Not applicable
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