MSP10
Membrane Scaffold Protein 1FC
recombinant, expressed in E. coli, buffered aqueous solution
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| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
5 mg | Per conoscere la disponibilità, visualizza il carrello | CHF 877.00 |
Informazioni su questo articolo
NACRES:
NA.26
UNSPSC Code:
12352200
Form:
buffered aqueous solution
Assay:
≥90% (SDS-GE)
Recombinant:
expressed in E. coli
Servizio Tecnico
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Permettici di aiutartirecombinant
expressed in E. coli
Quality Segment
description
C-terminal FLAG tagged
assay
≥90% (SDS-GE)
form
buffered aqueous solution
shipped in
ambient
storage temp.
−20°C
General description
Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The 1FC variant of MSP1D1 (MSP1FC) includes a C-terminal FLAG® tag.
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The 1FC variant of MSP1D1 (MSP1FC) includes a C-terminal FLAG® tag.
Application
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
Legal Information
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
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Questo articolo | |||
|---|---|---|---|
| assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) | assay ≥90% (SDS-GE) |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant expressed in E. coli |
| form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form buffered aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Classe di stoccaggio
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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