The use of a clean-up kit for plant growth media samples, considering that they are not a validated sample type for Methionine testing is recommended. Dilute the sample, likely at least 1:1000, to mitigate any matrix effects, with potential adjustments to the dilution based on performance at this ratio.
MAK347
Methionine Assay Kit (Fluorometric)
sufficient for 100 fluorometric tests
Sinonimo/i:
Methionine Fluorescence Detection Kit
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About This Item
Codice UNSPSC:
12161503
NACRES:
NA.84
Prodotti consigliati
impiego
sufficient for 100 fluorometric tests
Metodo di rivelazione
fluorometric
Malattie correlate
cardiovascular diseases
Temperatura di conservazione
−20°C
Descrizione generale
Methionine (Met) is one of the twenty proteogenic amino acids encoded by the genetic code, and one of nine essential amino acids, meaning it cannot be synthesized in vivo and must be obtained from dietary sources. Met is one of two sulfur-containing amino acids (the other being cysteine) and can serve as a precursor in cysteine biosynthesis. S-adenosylmethionine, another product of Met metabolism, is an important co-factor and methyl donor in a number of biological reactions such as methylation and polyamine synthesis. Met is also an intermediate species in the synthesis of phospholipids such as lecithin, and other small precursor molecules including taurine, an aminosulfonic acid. Oxidation of Met generates methionine sulfoxide, a species whose concentration tends to increase with age and is known to cause protein mis-folding and loss-of-function of numerous enzymes. This version of the amino acid can be restored back to methionine by another enzyme, methionine sulfoxide reductase. Biologically, Met levels can affect important events such as angiogenesis, protein synthesis, and cartilage production.
Caratteristiche e vantaggi
Compatible with high-throughput handling systems.
Compatibilità
Suitable for the determination of methionine concentration in serum, plasma, cell cultures and tissue lysates
Principio
The Methionine Assay Kit utilizes an enzymatic mechanism by which metabolism of methionine is coupled with the stoichiometric generation of hydrogen peroxide. A probe generates fluorometric signal that can be quantified at Ex/Em: 535/587 nm. The assay shows greater than 10-fold specificity for methionine over cysteine and greater than 40-fold selectivity over other standard and non-standard amino acids. After utilization of a proprietary Sample Clean-Up Mix, the method is suitable for use in blood samples, and can detect concentrations as low as 0.5 μM, or 25 pmole Methionine.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Repr. 1B - Skin Sens. 1
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Punto d’infiammabilità (°F)
188.6 °F
Punto d’infiammabilità (°C)
87 °C
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Would the cleanup procedure and dilution of the sample be expected to help with the issue of high background fluorescence in the no-enzyme control samples for the product MAK347? Also, could the acidic pH of the samples (pH 5.7) be an issue, considering the medium's lack of buffering capacity?
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