M9400
β-Mannosidase from Helix pomatia
5-30 units/mL, ammonium sulfate suspension, crude extract
Sinonimo/i:
β-D-Mannoside mannohydrolase
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.32
Prodotti consigliati
Forma fisica
ammonium sulfate suspension
Qualità
crude extract
PM
94 kDa
Concentrazione
5-30 units/mL
Attività estranea
β-N-acetylglucosaminidase and α-mannosidase <1.0%
Temperatura di conservazione
2-8°C
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Categorie correlate
Descrizione generale
β-Mannosidase is a lysosomal enzyme that belongs to the glycosyl hydrolase family 2.
Applicazioni
β-Mannosidase from Helix pomatia has been used:
- in the digestion of glycans for enzymatic analysis
- in the treatment of labeled monogalactosyl Manno-oligosaccharides
- in non-mammalian glycosidase inhibition assays
Azioni biochim/fisiol
β-Mannosidase participates in cleaving the β(1–4)-linked mannose located at the nonreducing end of N-glycosylated proteins. It is involved in the polysaccharide degradation pathway. In humans, mutations in the gene are associated with β-mannosidosis, a lysosomal storage disease. Low levels of β-Mannosidase due to mutations also lead to nystagmus, an eye disorder associated with involuntary eye movements.
Definizione di unità
One unit will hydrolyze 1 μmole of p-nitrophenyl-β-D-mannopyranoside to p-nitrophenol and D-mannopyranoside per min at pH 4.0 at 25°C.
Stato fisico
Suspension in 3.0 M (NH4)2SO4 containing 10 mM sodium acetate, pH approx. 4.0
Inibitore
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I clienti hanno visto anche
Roneel Prakash et al.
Journal of plant physiology, 169(12), 1125-1133 (2012-06-05)
Cell walls of tomato fruit contain hemicellulosic mannans that may fulfill a structural role. Two populations were purified from cell walls of red ripe tomato tissue and named galactoglucomannan-glucuronoxylan I and II (GGM-GX I and II), respectively. Both polysaccharides not
Pengfu Liu et al.
Applied biochemistry and biotechnology, 167(1), 73-80 (2012-04-25)
A novel biosensor strain (Escherichia coli ALM403) that responded to N-acyl homoserine lactone (AHL) was constructed using a luxR-Plux cassette as a regulatory sequence and β-mannanase as a reporter gene. Dinitrosalicylic acid method was used to detect the response of
A A Klyosov et al.
Carbohydrate research, 352, 65-69 (2012-03-23)
Statistical modeling was applied for describing structural features of β-(1→4)-D-galactomannans. According to the model suggested theoretical ratios of limiting degrees of locust bean, tara gum and guar gum galactomannan conversions by two β-(1→4)-mannanases of different origin (Myceliophthora thermophila and Trichoderma
P Wongputtisin et al.
Journal of applied microbiology, 113(4), 798-806 (2012-07-14)
To screen and select the Bacillus spp. from Tua-nao of northern Thailand for fermented corticate soybean meal (FCSBM) production. After isolation of Bacillus spp. from Tua-nao was carried out, cellulase, hemicellulases (i.e., β-mannanase and xylanase) and phytase production by isolated
Farage H Al-Ghazzewi et al.
Journal of the science of food and agriculture, 92(11), 2394-2396 (2012-04-13)
Glucomannan polysaccharides may be hydrolysed to lower molecular weight molecules using acids or enzymes, specifically mannanases or cellulases. Mannanases (β-mannanases) hydrolyse β-(1-4)-linked mannose residues randomly in mannans whilst cellulases (β-glucanase) hydrolyse β-(1-4)-linked glucose residues. The molecular weight of the hydrolysate
Articoli
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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