This product is methylated Jurkat DNA (T cell leukemia). Unfortunately, we do not evaluate the molecular weight of the product. However, it's genomic DNA.
M8570
Methylated Control DNA
50 ng/μL
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Informazioni su questo articolo
NACRES:
NA.55
UNSPSC Code:
23201100
Form:
liquid
Storage temp.:
−20°C
form
liquid
concentration
50 ng/μL
technique(s)
whole genome amplification: suitable
color
colorless
storage temp.
−20°C
General description
Fully methylated Jurkat DNA. 5-methyl-cytidine (5mC) content determined by mass spectrometry. See lot specific certificate of analysis or vial label for 5mC content of each lot.
Application
Methylated Control DNA has been used as a negative control in bisulfite sequencing of embryonic genomic DNA.
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Questo articolo | |||
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| description 50 ng/μL | description Useful as a control or standard for variety of epigenomic assays such as methylated or unmethylated DNA enrichment, bisulfite conversion or melt curve analysis to determine the status of global or locus- specific DNA methylation. | description The CpGenome Universal Methylated Mouse DNA Standard contains universally methylated mouse genomic DNA isolated from male Balb/c mice & enzymatically methylated at all CpGs by M.SssI methyltransferase. | description CpGenome 5-hmC DNA St&ard contains a linear, double-str&ed DNA (897 bp) with 100% hydroxymethylated cytosine. This st&ard can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications. |
| form liquid | form - | form - | form - |
| storage temp. −20°C | storage temp. - | storage temp. - | storage temp. - |
| color colorless | color - | color - | color - |
| concentration 50 ng/μL | concentration - | concentration - | concentration - |
Classe di stoccaggio
12 - Non Combustible Liquids
flash_point_f
No data available
flash_point_c
No data available
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Hello. Is this product a whole genomic DNA? Is it fragmented?
1 answer-
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What buffer is the Methylated Control DNA dissolved in?
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This product is dissolved in a TE buffer consisting of 10 mM Tris-HCl with 1 mM EDTA.
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It has been written 50 ng/ul for concentration. What is the total volume (as ul or ml) and total probe amount (as ug or mg) in the vial. Could you inform me, please
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The total volume is 16ul.
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What is the percentage of 5-Methylcytosine?
1 answer-
As mentioned on the product detail page, the % 5mC content is reported in the lot specific Certificate of Analysis, as well as the product label. The specification for 5mC is >/= 4.5%. The historical average is approximately 6.3%. Please see the sample Certificate of Analysis: https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/M8570-1VLSLCK7031.pdf
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